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Rate of segregation due to plasmid incompatibility

Published online by Cambridge University Press:  14 April 2009

John Cullum  and
Paul Broda
John Cullum
Affiliation:
Department of Molecular Biology, Kings Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland
Paul Broda
Affiliation:
Department of Molecular Biology, Kings Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland

Summary

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We calculated the rates of segregation due to plasmid incompatibility under several simple models. A common feature of all the models that we considered is that incompatibility is caused by the inability of the segregation mechanism to distinguish between two incompatible plasmids.

We measured the rate of segregation due to incompatibility of a pair of ColE1 derivatives under two conditions: (1) One plasmid was introduced into cells carrying the other by conjugation. (2) Cells carrying both plasmids were maintained by selection and then selection was released.

Interpretation of the results was made more difficult by effects of the Plasmids on the host cell's growth rate. These experiments gave results in agreement with the predictions of a random pool replication model. Published results were also in reasonable agreement with this model.

Type
Research Article
Information
Genetics Research , Volume 33 , Issue 1 , February 1979 , pp. 61 - 79
Copyright
Copyright © Cambridge University Press 1979

References

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