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Rapid detection of allelic recombination at the gall locus in yeast by assay of the recombinant gene product

Published online by Cambridge University Press:  14 April 2009

Leland H. Johnston
Leland H. Johnston
Affiliation:
Division of Microbiology, National Institute for Medical Research, Mill Hill, London NW7 1AA, England
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A diploid heteroallelic at the gal1 locus and producing little residual galactokinase activity was chosen. Following either γ- or UV-irradiation, recombination occurred leading to the formation of wild-type GAL1 genes and an increase in detectable galactokinase. An enhanced level of enzyme was first detectable 2 h after irradiation and reached a maximum within 12–14 h. With increasing doses of irradiation, more enzyme was produced and this increase superficially resembled that obtained for plated recombinants. However, far more enzyme was actually synthesized than expected from the number of viable recombinants in the cells assayed. This suggests that recombination must have occurred in cells without colony-forming ability, indeed it may have occurred more frequently in these cells than in viable cells.

Type
Research Article
Information
Genetics Research , Volume 39 , Issue 1 , February 1982 , pp. 85 - 97
Copyright
Copyright © Cambridge University Press 1982

References

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