Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-30T23:45:49.951Z Has data issue: false hasContentIssue false

A rapid and efficient replica plating technique

Published online by Cambridge University Press:  14 April 2009

Thomas H. Wood
Affiliation:
Department of Physics, University of Pennsylvania, Philadelphia Pa. 19104
Suresh K. Mahajan
Affiliation:
Department of Physics, University of Pennsylvania, Philadelphia Pa. 19104
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A new type of apparatus for replica plating is described, which reduces operator error and fatigue. Several replica plates can be made immediately, thus eliminating the need for a master plate in many cases.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1970

References

REFERENCES

Curtiss, R. & Stallions, D. R. (1969). Probability of integration and frequency of stable Hfr donors in F+ populations of Escherichia coli K-12. Genetics 63, 2738.CrossRefGoogle ScholarPubMed
Jacob, F. & Wollman, E. L. (1961). Sexuality and the Genetics of Bacteria, pp. 6465. New York: Academic Press.Google Scholar
Lederberg, J. & Lederberg, E. M. (1952). Replica plating and indirect selection of bacterial mutants. Journal of Bacteriology 63, 399406.CrossRefGoogle ScholarPubMed
Roberts, C. F. (1959). A replica plating technique for the isolation of nutritionally exacting mutants of a filamentous fungus (Aspergillus nidulans). Journal General Microbiology 20, 540548.CrossRefGoogle ScholarPubMed
Braendle, D. H. & Szybalski, W. (1957). Genetic interaction among streptomycetes: Heterokaryosis and synkaryosis. Proceedings National Academy of Sciences, Washington 43, 947955.CrossRefGoogle ScholarPubMed