Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-25T16:30:12.126Z Has data issue: false hasContentIssue false
  • English
  • Français

Protein and nucleic acid metabolism in organs from mice selected for larger and smaller body size

Published online by Cambridge University Press:  14 April 2009

G. C. Priestley  and
Moira S. M. Robertson
G. C. Priestley
Affiliation:
Agricultural Research Council Unit of Animal Genetics, Institute of Animal Genetics, Edinburgh, EH9 3JN, Scotland
Moira S. M. Robertson
Affiliation:
Agricultural Research Council Unit of Animal Genetics, Institute of Animal Genetics, Edinburgh, EH9 3JN, Scotland
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Studies of the growth and composition of Q-strain mice selected over 20 generations for high and low body weight at 6 weeks of age, and their unselected controls, were made on livers and kidneys of males from the five selection replicates A, B, C, D and F. Differences in growth rate between Large and Small QD mice were confirmed from 2 to 9 weeks of age, but were greatest in the third, fourth, sixth and seventh weeks. Total amounts of dry matter, protein, free amino acids, bulk RNA and ribosomes were increased or decreased from control values in proportion to organ weight. A less-perfect relationship between DNA content and organ weight suggested that some small changes in average cell mass had accompanied the main change in cell number in organs from the selected lines. Absorbance profiles of polyribosomes from both organs were identical in selected and control mice: selection had not operated on the proportion of single (currently inactive) ribosomes. Attempts to relate the observed differences in growth rate in QD mice to differences in the rate of protein synthesis produced an unexpected result: incorporation of radioactively labelled amino acids was consistently higher in the organs of the Small mice. Measurements of rates of protein turnover, and calculated rates of protein degradation, suggested that protein might also be degraded more rapidly in the small mice.

Type
Research Article
Information
Genetics Research , Volume 22 , Issue 3 , December 1973 , pp. 255 - 278
Copyright
Copyright © Cambridge University Press 1973

References

REFERENCES

Baird, D., Nalbandov, A. V. & Norton, H. W. (1952). Some physiological causes of genetically different rates of growth in swine. Journal of Animal Science 11, 292–300.CrossRefGoogle Scholar
Baserga, R., Petersen, R. O. & Estensen, R. D. (1966). RNA synthesis in liver and heart of growing and adult mice. Biochimica et Biophysica Acta 129, 259–270.CrossRefGoogle Scholar
Blobel, G. & Potter, V. R. (1967). Ribosomes in rat liver: an estimate of the percentage of free and membrane-bound ribosomes interacting with messenger RNA in vivo. Journal of Molecular Biology 28, 539–542.CrossRefGoogle ScholarPubMed
Bucher, N. L. R. & Malt, R. A. (1971). Regeneration of liver and kidney. Boston, U.S.A.: Little, Brown.Google Scholar
Burton, K. (1956). A study of the condition and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemical Journal 62, 315–323.CrossRefGoogle ScholarPubMed
Clark, J. N. (1969). Studies on the genetic control of growth in mice. Ph.D. Thesis, University of Edinburgh.Google Scholar
Chen, H. W., Hamer, D. H., Heiniger, H. & Meier, H. (1972). Stimulation of hepatic RNA synthesis in dwarf mice by ovine prolactin. Biochimica et Biophysica Acta 287, 90–97.CrossRefGoogle ScholarPubMed
Doljanski, F. (1960). The growth of the liver with special reference to mammals. International Review of Cytology 10, 217–241.Google Scholar
Epstein, C. J. (1967). Cell size, nuclear content, and the development of polyploidy in the mammalian liver. Proceedings National Academy of Sciences (U.S.A.) 57, 327–334.CrossRefGoogle ScholarPubMed
Falconer, D. S. (1960). Introduction to Quantitative Genetics. Edinburgh and London: Oliver and Boyd.Google Scholar
Falconer, D. S. (1974). Replicated selection for body weight in mice. Genetical Research 22, 291–321.CrossRefGoogle Scholar
Fowler, R. E. (1958). The efficiency of food utilization, digestibility of foodstuffs and energy expenditure of mice selected for large or small body size. Genetical Research 3, 51–68.CrossRefGoogle Scholar
Haschemeyer, A. E. V. & Gross, J. (1967). Isolation of liver polyribosomes in high yield. Biochimica et Biophysica Acta 145, 76–81.CrossRefGoogle ScholarPubMed
Korner, A. (1968). Anabolic action of growth hormone. Annals of the New York Academy of Sciences 148, 408–418.CrossRefGoogle ScholarPubMed
Lepore, P. D., Siegel, P. B. & Siegel, H. S. (1965). Nucleic acid composition of chicks and chick tissues from growth selected lines of White Rocks. Poultry Science 44, 126–130.CrossRefGoogle ScholarPubMed
Leuchtenberger, C., Helweg-Larsen, H. F. & Murmanis, L. (1954). Relationship between hereditary pituitary dwarfism and the formation of multiple desoxyribose nucleic acid (DNA) classes in mice. Laboratory Investigation 3, 245–260.Google ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Moore, S. & Stein, W. H. (1948). Photometric ninhydrin method for use in the chromatography of amino acids. Journal of Biological Chemistry 176, 367–388.CrossRefGoogle ScholarPubMed
Munro, H. N. & Fleck, A. (1966). Recent developments in the measurement of nucleic acids in biological materials. Analyst 91, 78–88.CrossRefGoogle ScholarPubMed
Nalbandov, A. V. (1963). Symposium on growth: endocrine causes of growth and growth stasis. Journal of Animal Science 22, 558–560.CrossRefGoogle Scholar
Priestley, G. C. & Malt, R. A. (1968). Development of the metanephric kidney: protein and nucleic acid synthesis. Journal of Cell Biology 37, 703–715.CrossRefGoogle ScholarPubMed
Priestley, G. C. & Malt, R. A. (1969). Membrane-bound ribosomes in kidney: methods of estimation and effect of compensatory growth. Journal of Cell Biology 41, 886–893.CrossRefGoogle Scholar
Priestley, G. C. & Robertson, M. (1972). Nucleic acids and polyribosomes in organs from mice with genetically different growth capacities. Journal of Cell Biology 55, 208a.Google Scholar
Roberts, R. C. (1966). The limits of artificial selection for body weight in the mouse. I. The limits attained in earlier experiments. Genetical Research 8, 347–360.CrossRefGoogle ScholarPubMed
Robertson, F. W. (1959). Studies in quantitative inheritance. XIII. Interrelations between genetic behaviour and development in the cellular constitution of the Drosophila wing. Genetics 44, 1113–1130.CrossRefGoogle ScholarPubMed
Robinson, D. W. & Bradford, G. E. (1969). Cellular response to selection for rapid growth in mice. Growth 33, 221–229.Google ScholarPubMed
Schimke, R. T., Ganschow, R., Doyle, D. & Arias, I. M. (1968). Regulation of protein turnover in mammalian tissues. Federation Proceedings 27, 1223–1230.Google ScholarPubMed
Scornick, O. A. (1972). Decreased in vivo disappearance of labelled liver protein after partial hepatectomy. Biochemical and Biophysical Research Communication 47, 1063–1066.CrossRefGoogle Scholar
Swick, R. W. (1958). Measurement of protein turnover in rat liver. Journal of Biological Chemistry 231, 751–764.CrossRefGoogle ScholarPubMed
Tomashefsky, P. & Tannenbaum, M. (1970). Macromolecular metabolism in renal compensatory hypertrophy. II. Protein turnover. Laboratory Investigation 23, 190–195.Google ScholarPubMed
Vendrely, R. (1955). The desoxyribonucleic acid content of the nucleus. In The Nucleic Acids vol. 2 (ed. Chargaff, E. and Davidson, J. N.), pp. 155–180. New York: Academic Press.Google Scholar
Wilson, S. H. & Hoagland, M. B. (1965). Studies on the physiology of rat liver polyribosomes: quantitation and intracellular distribution of ribosomes. Proceedings National Academy of Science(U.S.A.) 54, 600–607.CrossRefGoogle Scholar