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Production and modification of Mu (G–) phage particles in E. coli K12 and Erwinia

Published online by Cambridge University Press:  14 April 2009

Ariane Toussaint  and
Eric Schoonejans
Ariane Toussaint
Affiliation:
Laboratoire de Génétique, Université libre de Bruxelles, rue des Chevaux, 67, B 1640 Rhode St Genèse, Belgium
Eric Schoonejans
Affiliation:
Laboratoire de Génétique, Université libre de Bruxelles, rue des Chevaux, 67, B 1640 Rhode St Genèse, Belgium
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Summary

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We studied the amount of Mu(G+) and Mu(G−) phages in different Mu lysates prepared either upon induction or upon infection of E. coli and Erwinia strains. We also looked at the level of expression of the modification function (mom) by Mu(G−) phages, both after induction and after infection of E. coli and Erwinia. The expression of mom seems to be regulated in the same manner in E. coli and in the strain of Erwinia carotovora tested. The proportion of both types of Mu(G+) and Mu(G−) phages in induced lysates is very variable and we found growth conditions favouring the production of Mu(G−) particles. This should extend the use of Mu as a genetic tool and as a generalized transducing phage to many enterobacteria.

Type
Research Article
Information
Genetics Research , Volume 41 , Issue 2 , April 1983 , pp. 145 - 154
Copyright
Copyright © Cambridge University Press 1983

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