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Primary non-random X-inactivation caused by controlling elements in the mouse demonstrated at the cellular level

Published online by Cambridge University Press:  14 April 2009

Sohaila Rastan
Sohaila Rastan
Affiliation:
MRC Radiobiology Unit, Harwell, Didcot, Oxon, OX11 0RD
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Previous studies have shown that different alleles of the mouse X chromosomal controlling element locus, Xce, cause non-random X-chromosome inactivation as judged by variegation in the coats of female mice heterozygous for X-linked coat colour/structure genes, or Cattanach's translocation (Is (X; 7) Ct), or the relative activity of biochemical variants of the X-linked enzyme PGK. This paper presents evidence using the Kanda differential staining method on 6½ d.p.c. and 13½ d.p.c. female mouse embryos heterozygous for the marker X chromosome Is (X; 7) Ct and carrying different Xce alleles, that the Xce locus affects the randomness of X chromosome inactivation. Furthermore the fact that a marked Xce effect is demonstrable in female embryos as early as 6½ d.p.c. (i.e. very soon after the initial time of X-inactivation) is strong evidence that the Xce locus exerts its effect by causing primary non-random X-inactivation rather than by cell selection after initial random X-inactivation.

Type
Research Article
Information
Genetics Research , Volume 40 , Issue 2 , October 1982 , pp. 139 - 147
Copyright
Copyright © Cambridge University Press 1982

References

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