Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-24T07:46:46.788Z Has data issue: false hasContentIssue false
  • English
  • Français

Polymorphism and linkage analysis of the prothoracicotropic hormone gene in the silkmoth, Bombyx mori

Published online by Cambridge University Press:  14 April 2009

Toru Shimada ,
Tsuyoshi Hasegawa ,
Kayoko Matsumoto ,
Noriaki Agui  and
Masahiko Kobayashi
Toru Shimada
Affiliation:
Department of Agrobiology, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan
Tsuyoshi Hasegawa*
Affiliation:
Department of Agrobiology, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan
Kayoko Matsumoto
Affiliation:
Department of Agrobiology, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan
Noriaki Agui
Affiliation:
Department of Medical Entomology, National Institute of Health, Toyama 1-23-1, Shinjuku-ku, Tokyo 162, Japan
Masahiko Kobayashi
Affiliation:
Department of Agrobiology, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan
*
† Corresponding author: Dr Toru Shimada, Laboratory of Sericultural Science, Department of Agrobiology, Faculty of Agriculture, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113, Japan.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

We looked for polymorphism of the prothoracicotropic hormone gene locus (Ptth) among inbred strains of the silkmoth, Bombyx mori, by in vitro DNA amplification (polymerase chain reaction), and found three alleles, PtthA, PtthB and PtthC. The PtthA allele contained a third intron consisting of 680 bp and a fourth intron of 350 bp. PtthB contained the same size third intron but a longer fourth intron of 490 bp, while Ptthc had a longer third intron of 1080 bp and a shorter fourth intron of 350 bp. In 29 strains which we examined, 9 strains had PtthA, 8 strains had PtthB, and 2 strains had Ptthc. The other 10 strains had heterogeneous genotypes with the same 3 alleles.

Type
Research Article
Information
Genetics Research , Volume 63 , Issue 3 , June 1994 , pp. 189 - 195
Copyright
Copyright © Cambridge University Press 1994

References

Banno, Y., Kawaguchi, Y. & Doira, H. (1985). Genetical studies of the N-methyl-N-nitrosourea induced ‘non-molting b’ mutation in Bombyx mori. Journal of Sericultural Science of Japan 54, 227–231 (in Japanese).Google Scholar
Bender, W., Spierer, P. & Hogness, D. S. (1983). Chromosome walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster. Journal of Molecular Biology 168, 17–33.CrossRefGoogle ScholarPubMed
Doira, H. (1983). Linkage maps of Bombyx mori—status quo in 1983. Sericologia 23, 245–269.Google Scholar
Doira, H., Chikushi, H. & Kihara, H. (1978). Linkage studies of Bombyx mori: Discovery of a new linkage group or-mw. Journal of Sericultural Science of Japan 47, 27–31 (in Japanese).Google Scholar
Doira, H., Kihara, H. & Banno, Y. (1984). Genetical studies on the ‘non-molting dwarf’ mutations in Bombyx mori. Journal of Sericultural Science of Japan 53, 427–431 (in Japanese).Google Scholar
Doira, H., Fujii, H., Kawaguchi, Y., Kihara, H. & Banno, Y. (1992). Genetical Stocks and Mutations of Bombyx mori: Important Genetic Resources. Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, 73 pp.Google Scholar
Hirobe, T. (1952). A preliminary report on inheritance of the recessive trimolter in the silkworm, Bombyx mori. Technical Data, Department of Sericulture, Ministry of Agriculture and Forestry, Japan 33, 23 (in Japanese).Google Scholar
Japanese Society of Sericultural Science (1986). Nomenclature and symbols of the genes in the silkworm, Bombyx mori. Journal of Sericultural Science of Japan 55, 95–111 (in Japanese).Google Scholar
Kataoka, H., Nagasawa, H., Isogai, A., Tamura, S., Mizoguchi, A., Fujiwara, Y., Suzuki, C., Ishizaki, H. & Suzuki, A. (1987). Isolation and partial characterization of prothoracicotropic hormone of the silkworm, Bombyx mori. Agricultural and Biological Chemistry 51, 1067–1076.Google Scholar
Kataoka, H., Nagasawa, H., Isogai, A., Ishizaki, H. & Suzuki, A. (1991). Prothoracicotropic hormone of the silkworm, Bombyx mori: amino acid sequence and dimeric structure. Agricultural and Biological Chemistry 55, 73–86.Google Scholar
Kawakami, A., Kataoka, H., Oka, T., Mizoguchi, A., Kimura-Kawakami, M., Adachi, T., Iwami, M., Nagasawa, H., Suzuki, A. & Ishizaki, H. (1990). Molecular cloning of the Bombyx mori prothoracicotropic hormone. Science 247, 1333–1335.Google Scholar
Morohoshi, S. (1957). Physiological Studies on Moltinism and Voltinism in Bombyx mori: A New Hormonal Balance Theory on the Growth. Japan Society for the Promotion of Science, Tokyo, Japan, 202 pp.Google Scholar
Nagata, M., Tsuchida, K., Shimizu, K. & Yoshitake, N. (1987). Physiological aspects of nm-g mutant: an ecdysteroid-deficient mutant of the silkworm, Bombyx mori. Journal of Insect Physiology 33, 723–727.CrossRefGoogle Scholar
Nagatomo, Y. (1941). On the sex-dependent expression of the trimolting property. Journal of Sericultural Science of Japan 12, 171–183 (in Japanese).Google Scholar
Ninaki, O., Doira, H. & Chikushi, H. (1980). Genetical studies of the ‘dimolting’ mutant in Bombyx mori. Journal of Sericultural Science of Japan 49, 347–351 (in Japanese).Google Scholar
Ogura, S. (1931). Erblichkeitsstudien am Seidenspinner Bombyx mori L. I. Genetische Untersuchung der Kokonfarbe. Zeitschrift für induktiv Abstammungs-und Vererbungslehre 58, 122–156.Google Scholar
Ogura, S. (1932). Erblichkeitsstudien am Seidenspinner Bombyx mori L. II. Genetische Untersuchung der Hautung. Zeitschrift für induktiv Abstammungs-und Vererbungslehre 61, 315–408.Google Scholar
Ogura, S. (1933). Erblichkeitsstudien am Seidenspinner Bombyx mori L. III. Genetische Untersuchung der Hautung. Zeitschrift für induktiv Abstammungs-und Vererbungslehre 64, 205–268.Google Scholar
Oota, S., Watanabe, A. & Tokunaga, H. (1957). Genetical study on a spontaneous mutant, two molter, in the silkworm, Bombyx mori. Journal of Sericultural Science of Japan 26, 77–81 (in Japanese).Google Scholar
Sambrook, J., Frisch, E. F. & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd Ed.New York: Cold Spring Harbor Laboratory Press.Google Scholar
Sanger, F., Miklen, S. & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74, 5463–5467.Google Scholar
Shimizu, K., Enokijima, M., Fujimaki, T., Fujimori, H. & Matsuno, M. (1983). Inheritance of a new mutant, ‘Matsuno non-molting’ in Bombyx mori. Journal of Sericultural Science of Japan 52, 348–353 (in Japanese).Google Scholar
Shimizu, K., Tanaka, N. & Matsuno, M. (1980). Linkage analysis of a non-molting mutant of Bombyx mori and its application to the stock maintenance. Journal of Sericultural Science of Japan 49, 7–12 (in Japanese).Google Scholar
Shimodaira, M. (1947). Studies of linkage in the silkworm. I. Relation between VI and VIII linkage group. Japanese Journal of Genetics 22, 82–84 (in Japanese).Google Scholar
Umeya, Y. & Karasawa, Y. (1930). A silkworm strain with a factor inhibiting development. Japanese Journal of Genetics 6, 188–194 (in Japanese).Google Scholar
Yokoyama, J. (1936). Histological observations on a nonmolting strain of silkworm. Proceedings of the Royal Entomological Society, London (A) 11, 35–44.Google Scholar
Yoshitake, N. (1968). Phylogenetic aspects of the origin of Japanese race of the silkworm, Bombyx mori L. Journal of Sericultural Science of Japan 37, 83–87 (in Japanese).Google Scholar
Yoshitake, N., Kobayashi, M. & Miyashita, T. (1987 a). On the ‘skunk’ mutant in the silkworm. Journal of Sericultural Science of Japan 47, 32–34 (in Japanese).Google Scholar
Yoshitake, N., Kobayashi, M. & Ogawa, Y. (1987 b). On a smell factor existing in faeces from the skunk silkworm, Bombyx mori. Journal of Sericultural Science of Japan 47, 161–165 (in Japanese).Google Scholar