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Polymorphism and linkage analysis of the prothoracicotropic hormone gene in the silkmoth, Bombyx mori

Published online by Cambridge University Press:  14 April 2009

Toru Shimada ,
Tsuyoshi Hasegawa ,
Kayoko Matsumoto ,
Noriaki Agui  and
Masahiko Kobayashi
Toru Shimada
Affiliation:
Department of Agrobiology, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan
Tsuyoshi Hasegawa*
Affiliation:
Department of Agrobiology, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan
Kayoko Matsumoto
Affiliation:
Department of Agrobiology, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan
Noriaki Agui
Affiliation:
Department of Medical Entomology, National Institute of Health, Toyama 1-23-1, Shinjuku-ku, Tokyo 162, Japan
Masahiko Kobayashi
Affiliation:
Department of Agrobiology, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan
*
† Corresponding author: Dr Toru Shimada, Laboratory of Sericultural Science, Department of Agrobiology, Faculty of Agriculture, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113, Japan.
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Summary

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We looked for polymorphism of the prothoracicotropic hormone gene locus (Ptth) among inbred strains of the silkmoth, Bombyx mori, by in vitro DNA amplification (polymerase chain reaction), and found three alleles, PtthA, PtthB and PtthC. The PtthA allele contained a third intron consisting of 680 bp and a fourth intron of 350 bp. PtthB contained the same size third intron but a longer fourth intron of 490 bp, while Ptthc had a longer third intron of 1080 bp and a shorter fourth intron of 350 bp. In 29 strains which we examined, 9 strains had PtthA, 8 strains had PtthB, and 2 strains had Ptthc. The other 10 strains had heterogeneous genotypes with the same 3 alleles.

Type
Research Article
Information
Genetics Research , Volume 63 , Issue 3 , June 1994 , pp. 189 - 195
Copyright
Copyright © Cambridge University Press 1994

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