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The plasmid curing action of imipramine in Escherichia coli K12

Published online by Cambridge University Press:  14 April 2009

J. Molnár ,
I. Béládi  and
I. B. Holland
J. Molnár
Affiliation:
Institute of Microbiology, University Medical School, Szeged, Hungary
I. Béládi
Affiliation:
Institute of Microbiology, University Medical School, Szeged, Hungary
I. B. Holland
Affiliation:
Department of Genetics, School of Biological Sciences, University of Leicester, Leicester, England
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Summary

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Curing of an F-prime plasmid by imipramine was most efficient on bacteria growing semianaerobically at 37 °C. The plasmid curing effect of imipramine was increased in the presence of methylene blue, whilst fluorescein, chlorpromazine-sulphoxide and tetraoxyanthrachinon antagonized the plasmid curing action of the drug. In addition to its plasmid curing effect, imipramine treatment selected for Lon mutants at high frequency. Lon mutants show increased resistance to the drug but cured strains, if Lon+, are not resistant.

Type
Short Papers
Information
Genetics Research , Volume 31 , Issue 2 , April 1978 , pp. 197 - 201
Copyright
Copyright © Cambridge University Press 1978

References

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