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The phasmid as a tool for plasmid genetics: I. Fine structure of the β-lactamase gene

Published online by Cambridge University Press:  14 April 2009

Luisa Castagnoli ,
Gianni Cesareni  and
Sydney Brenner
Luisa Castagnoli
Affiliation:
MRC Laboratory of Molecular Biology, University Postgraduate Medical School, Hills Road, Cambridge CB2 2QH, England
Gianni Cesareni
Affiliation:
MRC Laboratory of Molecular Biology, University Postgraduate Medical School, Hills Road, Cambridge CB2 2QH, England
Sydney Brenner
Affiliation:
MRC Laboratory of Molecular Biology, University Postgraduate Medical School, Hills Road, Cambridge CB2 2QH, England
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The reversible integration of a plasmid into a phage genome via int-mediated recombination permits rapid and facile analysis of plasmid genes. Seventy-six independent mutants in the β-lactamase (bla) gene have been isolated and genetically mapped. The end points of deletions generated by partial digestion with restriction enzymes have been identified and this, together with the assignment of some nonsense mutations to specific amino acid residues, allows alignment of the genetic map with the DNA sequence.

Type
Research Article
Information
Genetics Research , Volume 40 , Issue 3 , December 1982 , pp. 217 - 231
Copyright
Copyright © Cambridge University Press 1982

References

REFERENCES

Achtman, M., Manning, P. A., Edelbluth, C. & Herrlich, P. (1979). Export without proteolytic processing of inner and outer membrane proteins by F sex factor tra cistrons in Escherichia coli minicells. Proceedings of the National Academy of Sciences of the USA 76, 4837–4841.CrossRefGoogle ScholarPubMed
Ambler, R. P. & Scott, G. K. (1978). Partial amino acid sequence of penicillinase by Escherichia coli plasmid R6K. Proceedings of the National Academy of Sciences of the USA 75, 3732–3736.CrossRefGoogle ScholarPubMed
Backman, K., Betlach, M., Boyer, H. W. & Yanofsky, S. (1978). Genetic and physical studies on the replication of ColEl — type plasmids. Cold Spring Harbor Symposia on Quantitative Biology 43, 69–76.CrossRefGoogle Scholar
Bassford, P. & Beckwith, J. (1979). Escherichia coli mutants accumulating the precursor of a secreted protein in the cytoplasm. Nature 277, 538–541.CrossRefGoogle ScholarPubMed
Bazarel, M. & Helinsky, D. R. (1970). Replication of a bacterial plasmid and an episome in Escherichia coli. Biochemistry 9, 399–406.CrossRefGoogle Scholar
Brenner, S., Cesareni, G. & Karn, J. (1981). Phasmids: hybrids between ColE1 plasmids and bacteriophage lambda. Gene 17, 27–44.CrossRefGoogle Scholar
Clewell, D. B. & Helinski, D. R. (1969). Supercoiled circular DNA protein complex in Escherichia coli: purification and induced conversion to an open circular DNA form. Proceedings of the National Academy of Sciences of the USA 62, 1159–1166.CrossRefGoogle Scholar
Collins, J., Yanofsky, S. & Helinski, D. R. (1978). Altered DNA protein relaxation complex in a replication mutant of plasmid ColE1. Molecular and General Genetics 167, 21–28.CrossRefGoogle Scholar
Coulondre, C. & Miller, J. H. (1977). Genetic studies of the lac represser. Journal of Molecular Biology 117, 525–567.CrossRefGoogle Scholar
Cox, E. C. & Yanofsky, C. (1967). Altered base ratios in the DNA of an Escherichia coli mutator strain. Proceedings of the National Academy of Sciences of the USA 58, 1895–1902.CrossRefGoogle ScholarPubMed
Datta, N. & Kontomichalou, P. (1965). Penicillinase synthesis controllled by infectious R factors in Enterobacteriaceae. Nature 208, 239–241.CrossRefGoogle ScholarPubMed
Dougan, G. & Sherrat, D. (1977). The transposon Tn1 as a probe for studying ColE1 structure and function. Molecular and General Genetics 151, 151–160.CrossRefGoogle ScholarPubMed
Emr, S. D., Schwartz, M. & Silhavy, T. J. (1978). Mutations altering the cellular location of the phage receptor, an Escherichia coli outer membrane protein. Proceedings of the National Academy of Sciences of the USA 75, 5802–5806.CrossRefGoogle ScholarPubMed
Georgopulos, C. P. & Herskowitz, I. (1971). Escherichia coli mutants blocked in lambda DNA synthesis. In The Bactiophage Lambda (ed. Hershey, A. D.), pp. 553–564. Cold Spring Harbor Laboratory.Google Scholar
Inselburg, J. (1977). Isolation mapping and examination of effects of insertion in ColE1 plasmids. Journal of Bacteriology 129, 482–491.CrossRefGoogle ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of bacteriophage T4. Nature 227, 680–685.CrossRefGoogle ScholarPubMed
Lederberg, E. M. & Cohen, S. N. (1974). Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. Journal of Bacteriology 119, 1071–1074.CrossRefGoogle ScholarPubMed
Levy, S. B. (1971). Physical and functional characteristics of R-factor deoxyribonucleic acid segregated into Escherichia coli minicells. Journal of Bacteriology 108, 300–308.CrossRefGoogle ScholarPubMed
Miller, J. H. (1972). Experiments in Molecular Genetics. Cold Spring Harbor Laboratory.Google Scholar
Miller, J. H., Ganem, D., Lu, P. & Schmitz, A. (1977). Genetic studies of the lac represser. Journal of Molecular Biology 109, 175–201.CrossRefGoogle Scholar
Miller, J. H., Coulondre, C. & Farabaugh, P. J. (1978). Correlation of nonsense sites in the laci gene with specific codons in the nucleotide sequence. Nature 274, 770–775.CrossRefGoogle ScholarPubMed
O'Callaghan, C. H., Morris, A., Kirby, S. & Shingler, A. H. (1972). Novel method for detection of β-lactamase, a chromogenic cephalosporin substrate. Antimicrobial Agents and Chemotherapy 1, 283–288.CrossRefGoogle ScholarPubMed
Oka, A. & Inselburg, J. (1975). Replicative intermediates of ColE1 plasmid DNA in minicells. Proceedings of the National Academy of Sciences of the USA 72, 829–833.CrossRefGoogle Scholar
Parkinson, J. S. & Davis, R. W. (1971). A physical map of the left arm of the lambda chromosome. Journal of Molecular Biology 56, 425–428.CrossRefGoogle ScholarPubMed
Roozen, K. J., Fenwick, R. G. Jr. & Curtiss, R. (1971). Synthesis of ribonucleic acid and protein in plasmid containing minicells of Escherichia coli K12. Journal of Bacteriology 107, 21–23.CrossRefGoogle Scholar
Sakakibara, Y. & Tomizawa, J. (1971). Replication of ColE1 plasmid DNA in bacterial extracts. Proceedings of the National Academy of Sciences of the USA 71, 802–806.CrossRefGoogle Scholar
Sutcliffe, J. G. (1978). Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proceedings of the National Academy of Sciences of the USA 75, 3737–3741.CrossRefGoogle ScholarPubMed
Tomizawa, J., Sakakibara, Y. & Kakefuda, T. (1975). Replication of ColE1 plasmid DNA added to cell extracts. Proceedings of the National Academy of Sciences of the USA 72, 1050–1054CrossRefGoogle Scholar