Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-25T16:54:52.392Z Has data issue: false hasContentIssue false
  • English
  • Français

p–Fluorophenylalanine-induced mitotic haploidization in Ustilago violacea

Published online by Cambridge University Press:  14 April 2009

A. W. Day  and
J. K. Jones
A. W. Day
Affiliation:
Department of Agricultural Botany, University of Reading
J. K. Jones
Affiliation:
Department of Agricultural Botany, University of Reading
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Haploid segregante from vegetative diploids of Ustilago violacea appear as spherical colonies (papillae) growing above a background of dead diploid cells on complete medium containing DL-p–fluorophenylalanine (PFP). Most mutants segregate normally but one-third of the mutants were expressed infrequently in 0–5% papillae only. These mutants, designated ‘missing-markers’, were found to be on either of two chromosomes that remained disomic after treatment with PFP. When cells from a disomic papillum were streaked on complete medium, monosomics in which ‘missing-markers’ were expressed segregated spontaneously at a low frequency. Thus, of 10–12 linkage groups identified in U. violacea, two remain disomic after PFP treatment. Possible reasons for these differences between chromosomes in the same genome are discussed.

Haploid and diploid stock cultures did not differ either in resistance to PFP, or in the production of papillae on PFP medium. Haploid segregante from a diploid were slightly more resistant to PFP than the wild-type haploid cultures under some conditions, but were very different in that they no longer produced papillae on PFP medium. These haploid segre-gants resembled one of three PFP-resistant mutants (pfp-A) isolated from a wild-type haploid stock grown on PFP medium. The significance of these results to the mechanism of haploidization in U. violacea is discussed.

Type
Research Article
Information
Genetics Research , Volume 18 , Issue 3 , December 1971 , pp. 299 - 309
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Burnett, J. H. (1968). Fundamentals of Mycology. London: Edward Arnold.Google Scholar
Da Cunha, M. F. (1970). Mitotic mapping of Schizosaccharomyces pombe. Genetical Research 16, 127–144.CrossRefGoogle Scholar
Day, A. W. & Jones, J. K. (1966). Induced haploidization in diploid cultures of Ustilago violacea. Microbial Genetica Bulletin, no. 25, pp. 5–6.Google Scholar
Day, A. W. & Jones, J. K. (1968). The production and characteristics of diploids in Ustilago violacea. Genetical Research 11, 63–81.CrossRefGoogle Scholar
Day, A. W. & Jones, J. K. (1969). Sexual and parasexual analysis of Ustilago violacea. Genetical Research 14, 195–221.CrossRefGoogle ScholarPubMed
Dorn, G. L. (1967). A revised map of the eight linkage groups of Aspergillus nidulans. Genetics, Princeton 56, 619–631.CrossRefGoogle ScholarPubMed
Ellingboe, A. H. (1964). Somatic recombination in dikaryon K of Schizophyllum commune. Genetics, Princeton 49, 247–251.CrossRefGoogle ScholarPubMed
Fjeld, A. & Strømnaes, O. (1966). The parasexual cycle and linkage groups in Penicillium expansum. Hereditas 54, 389–403.CrossRefGoogle ScholarPubMed
Gutz, H. (1966). Induction of mitotic segregation with p–fluorophenylalanine in Schizosaccharomyces pombe. Journal of Bacteriology 92, 1567–1568.CrossRefGoogle ScholarPubMed
Käfer, E. (1958). An 8-chromosome map of Aspergillus nidulans. Advances in Genetics 9, 105–145.CrossRefGoogle ScholarPubMed
Käfer, E. (1961). The processes of spontaneous recombination in vegetative nuclei of Aspergillus nidulans. Genetics, Princeton 46, 1581–1609.CrossRefGoogle ScholarPubMed
Lewis, L. A. (1969). Correlated meiotic and mitotic maps in Aspergillus amstelodami. Genetical Research 14, 185–195.CrossRefGoogle ScholarPubMed
Lewis, L. A. & Barrow, G. L. (1964). The pattern of the parasexual cycle in Aspergillus amstelodami. Genetical Research 5, 162–164.CrossRefGoogle Scholar
Lhoas, P. (1961). Mitotic haploidization by treatment of Aspergillus niger diploide with p–fluorophenylalanine. Nature, London 190, 744.CrossRefGoogle Scholar
Lhoas, P. (1967). Genetic analysis by means of the parasexual cycle in Aspergillus niger. Genetical Research 10, 45–61.CrossRefGoogle ScholarPubMed
Lhoas, P. (1968). Growth rate and haploidization of Aspergillus niger on medium containing p–fluorophenylalanine. Genetical Research 12, 305–315.CrossRefGoogle ScholarPubMed
McCully, K. S. & Forbes, E. (1965). The use of p–fluorophenylalanine with ‘master strains’ of Aspergillue nidulans for assigning genes to linkage groups. Genetical Research 6, 352–359.CrossRefGoogle ScholarPubMed
Millington-Ward, A. M. (1967). A vegetative instability in Aspergillus nidulans. Genetica 38, 191–207.CrossRefGoogle Scholar
Sinha, U. (1967). Aromatic amino acid biosynthesis and parafluorophenylalanine resistance in Aspergillus nidulans. Genetical Research 10, 261–272.CrossRefGoogle ScholarPubMed
Sisken, J. E. & Wilkes, E. (1967). The time of synthesis and the conservation of mitosis related proteins in cultured human amnion cells. Journal of Cell Biology 34, 97–110.CrossRefGoogle ScholarPubMed
Strømnaes, O. (1968). Genetic changes in Saccharomyces cerevisiae grown on media containing DL-p–fluorophenylalanine. Hereditas 59, 197–220.CrossRefGoogle Scholar
Strømnaes, O. & Garber, E. D. (1963). Heterocaryosis and the parasexual cycle in Aspergillus fumigatus. Genetics, Princeton 48, 653–662.CrossRefGoogle ScholarPubMed
Strømnaes, O., Garber, E. D. & Beraha, L. (1964). Genetics of phytopathogenic fungi. IX. Heterocaryosis and the parasexual cycle in Penicillium italicum and P. digitatum. Canadian Journal of Botany 42, 423–427.CrossRefGoogle Scholar