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Parameters in gene conversion: An algebraic analysis of the hybrid DNA model at the gray locus of Sordaria fimicola.*

Published online by Cambridge University Press:  14 April 2009

Angelos Kalogeropoulos  and
Pierre Thuriaux
Angelos Kalogeropoulos
Affiliation:
Laboratoire de Génétique, bâtiment 400, Centre d'Orsay Université de Paris-Sud. F 91405 Orsay Cedex, France
Pierre Thuriaux
Affiliation:
Laboratoire de Génétique, bâtiment 400, Centre d'Orsay Université de Paris-Sud. F 91405 Orsay Cedex, France Institut für allgemeine Mikrobiologie, Baltzerstrasse 4, CH-3012 Bern, Schweiz
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We have extended previous algebraic analyses of aberrant segregation at the gray locus of Sordaria fimicola (Whitehouse, 1965; Emerson, 1966; Fincham, Hill & Reeve, 1980) to the more complex situation where aberrant segregations are detected in three factor crosses involving two flanking markers. This algebra has been applied to seven gray alleles which have been extensively characterized for their pattern of gene conversion and postmeiotic segregation by Kitani & Olive (1967). It is based on seven major types of aberrant segregation which can be distinguished in the presence of flanking markers spanning the converting site, and allows us to use up to six parameters to describe hDNA formation and mismatch repair. We present solutions which predict a spectrum of aberrant segregation fitting the experimental data at the P > 0·05 level for six of the seven alleles tested. They are consistent with the following properties of hDNA at the gray locus: (1) the single stranded DNA transferred during hDNA formation has always the same chemical polarity. (2) hDNA is mostly, if not entirely, symmetric, and its probability of formation is constant over the whole gene. (3) Disparity in aberrant segregation is mostly, if not entirely due to disparity in mismatch repair.

Type
Research Article
Information
Genetics Research , Volume 40 , Issue 1 , August 1982 , pp. 1 - 18
Copyright
Copyright © Cambridge University Press 1982

References

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