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The Ontogeny of γ-crystallin mRNAs in CatFraser Mice

Published online by Cambridge University Press:  14 April 2009

Maciej Kuliszewski ,
Jim Rupert  and
R. Gold
Maciej Kuliszewski
Affiliation:
Department of Medical Genetics, University of Toronto, Toronto, Ontario, Canada M5S 1A8
Jim Rupert
Affiliation:
Department of Medical Genetics, University of Toronto, Toronto, Ontario, Canada M5S 1A8
R. Gold*
Affiliation:
Department of Medical Genetics, University of Toronto, Toronto, Ontario, Canada M5S 1A8
*
* Corresponding author.
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Summary

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Mice which are either homozygous or heterozygous for the CatFraser mutation have ocular cataracts accompanied by selective reduction of the γ-crystallins, a homologous family of proteins present in the lens and encoded by a family of tightly linked genes. We measured the concentrations of four different mRNAs, each encoding a different γ-crystallin, in the lenses of homozygous CatFraser mice and in normal controls at various stages of development by preparing Northern blots from lens RNA, probing with RNAs complementary to each of the four messages and densitometry of the bands thus generated. The results show that, for each of these messages, the ontogenetic patterns observed in normal mice are retained in the mutant, but at much lower concentrations.

Type
Research Article
Information
Genetics Research , Volume 52 , Issue 1 , August 1988 , pp. 45 - 49
Copyright
Copyright © Cambridge University Press 1988

References

Breitman, M., Lok, S., Wistow, G., Piatigorsky, J., Treton, J. A., Gold, R. & Tsui, L.-C. (1984). γ-crystallin family of the mouse lens: structural and evolutionary relationships. Proceedings of the National Academy of Science 81, 7762–7766.Google Scholar
Day, T. H. & Clayton, R. M. (1972). Multiple changes in lens protein composition associated with the CatFr gene in the mouse. Genetical Research 19, 241–249.Google Scholar
Delaye, M. & Tardieu, A. (1983). Short-range order of crystallin proteins accounts for eye lens transparency. Nature 302, 415–417.CrossRefGoogle ScholarPubMed
Fraser, C. & Schabtach, G. (1962). ‘Shrivelled’: a hereditary degeneration of the lens in the mouse. Genetical Research 19, 383–387.CrossRefGoogle Scholar
Garber, A. T. & Gold, R. (1982). Comparative two dimensional electrophoresis of water soluble proteins from bovine and murine lens. Experimental Eye Research 36, 585–596.Google Scholar
Garber, A. T., Stirk, L. & Gold, R. (1983). Abnormalities of crystallins in the lens of the CatFraser mouse. Experimental Eye Research 36, 165–169.Google Scholar
Garber, A. T., Winkler, C., Shinohara, T., King, C. R., Inana, G., Piatigorsky, J. & Gold, R. (1985). Selective loss of a family of gene transcripts in a hereditary murine cataract. Science 227, 74–77.Google Scholar
Hamai, Y. & Kuwabara, T. (1975). Early cytologic changes of the Fraser cataract. An electron microscopic study. Investigative Opthalmology 14, 517–527.Google Scholar
Harding, J. & Dilley, K. (1975). Structural proteins of the mammalian lens: A review with emphasis on changes in development, aging and cataract. Experimental Eye Research 22, 1–73.Google Scholar
Kador, P. F., Fukui, H., Fukusui, S., Jernigan, H. Jr & Kinoshita, J. (1980). Philly Mouse: a new model of hereditary cataract. Experimental Eye Research 30, 59–68.Google Scholar
Lok, S., Breitman, M., Cheplinsky, A., Piatigorsky, J., Gold, R. & Tsui, L.-C. (1985). Lens specific promoter activity of a mouse γ-crystallin gene. Molecular and Cellular Biology 5, 2221–2230.Google Scholar
Lok, S., Tsui, L.-C., Shinohara, T., Piatigorsky, J., Gold, R. & Breitman, M. L. (1984). Analysis of the mouse γ-crystallin gene family: assignment of multiple cDNAs to discrete genomic sequences and characterization of a representative gene. Nucleic Acids Research 12, 4517–4529.Google Scholar
Maniatis, T., Frisch, E. F. & Sambrook, J. (1982). In Molecular Cloning, A Laboratory Manual, Cold Spring Harbor.Google Scholar
Muggleton-Harris, A. L., Festing, M. F. W. & Hall, M. (1987). A gene location for the inheritance of the cataract Fraser (CatFr) mouse congenital cataract. Genetical Research 49, 235–238.Google Scholar
Murer-Orlando, M., Paterson, R., Lok, S., Tsui, L.-C. & Breitman, M. (1987). Differential regulation of γ-crystallin genes during mouse lens development. Developmental Biology 19, 260–267.CrossRefGoogle Scholar
Piatigorsky, J. (1981). Lens differentiation in vertebrates. A review of cellular and molecular features. Differentiation 19, 134–153.Google Scholar
Piatigorsky, J. (1984). Lens crystallins and their gene families. Cell 38, 620–621.CrossRefGoogle ScholarPubMed
Quinlan, P., Oda, S., Breitman, M. L. & Tsui, L.-C. (1987). The mouse eye lens obsolescence (Elo) mutant: Studies on crystallin gene expression and linkage analysis between the mutant locus and the γ-crystallin genes. Genes and Development 1, 637–644.CrossRefGoogle ScholarPubMed
Rupert, J., Kuliszewski, M., Tsui, L.-C., Breitman, M. L. & Gold, R. J. M. (1988). The murine cataractogenic mutation, CatFr, segregates independently of the γ-crystallin genes. Genetical Research (in press).Google Scholar
Sakurakawa, M., Kuwabara, T., Kinoshita, J. H. & Jukui, H. N. (1975). Swelling of the lens fibers. Experimental Eye Research 21, 381–394.Google Scholar
Shinohara, T. & Piatigorsky, J. (1980). Persistence of crystallin messenger RNAs with reduced translation in hereditary cataracts in mice. Science 210, 914–916.Google Scholar
Zigman, S. (1985). Selected aspects of lens differentiation. Biological Bulletin 168, 189–213.CrossRefGoogle Scholar
Zwann, J. & Williams, R. (1968). Morphogenesis of the eye lens in a mouse strain with hereditary cataracts. Experimental Zoology 169, 407–422.Google Scholar
Zwann, J. & Williams, R. (1969). Cataracts and abnormal proliferation of the lens epithelium in mice carrying the CatFr mutation. Experimental Eye Research 8, 161–167.Google Scholar