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Observed relationships between protein heterozygosity and protein genetic distance and comparisons with neutral expectations

Published online by Cambridge University Press:  14 April 2009

R. D. Ward  and
D. O. F. Skibinski
R. D. Ward
Affiliation:
Department of Human Sciences, Environmental Biology Unit, Loughborough University, Loughborough, Leicestershire, LE11 3TU, U.K.
D. O. F. Skibinski
Affiliation:
Department of Genetics, University College of Swansea, Singleton Park, Swansea, SA2 8PP, U.K.
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Relationships between protein genetic distance (D) and protein heterozygosity (H) were studied using allele frequency data for 42 proteins derived from multilocus electrophoretic surveys of genetic variation in over 200 invertebrate and over 300 vertebrate species. D¯ and H¯ values for the different proteins (mostly enzymes) were calculated, and large and significant correlations between D¯ and H¯ were found in comparisons of both intraspecific and interspecific populations. Empirical relationships between D¯ and H¯ were compared with neutral expectations under the stepwise model of neutral mutation with the assumption that populations are in equilibrium with respect to the effects of mutation and genetic drift.

At low divergence levels, a linear relationship of D¯ on H¯ was observed, but at high levels of divergence D tended towards an asymptote at high H¯. The results at high divergence cannot be explained using the approximate relationship D = 2ut (where u = mutation rate, t = time). However, computer simulations of neutral models showed that changes of this nature in the relationship between D¯ and H¯ were to be expected as divergence increases, the equation D = 2ut being a poor approximation at high D We therefore conclude that the observed relationships between D¯ and H¯ are, in fact, compatible with equilibrium neutral theory.

Type
Research Article
Information
Genetics Research , Volume 45 , Issue 3 , June 1985 , pp. 315 - 340
Copyright
Copyright © Cambridge University Press 1985

References

REFERENCES

Adams, S. E., Smith, M. H. & Baccus, R. (1980). Biochemical variation in the American alligator. Herpetologica 36, 289–296.Google Scholar
Andersson, L., Ryman, N., Rosenberg, R. & Stahl, G. (1981). Genetic variability in Atlantic herring (Clupea harengus harengus): description of protein loci and population data. Hereditas 95, 69–78.CrossRefGoogle Scholar
Ashton, R. E., Braswell, A. L. & Guttman, S. I. (1980). Electrophoretic analysis of three species of Necturus (Amphibia: Proteidae), and the taxonomic status of Necturus lewisi (Brimley). Brimleyana 4, 43–46.Google Scholar
Ayala, F. J., Valentine, J. W., Barr, L. G. & Zumwalt, G. S. (1974). Genetic variability in a temperate intertidal Phoronid, Phoronopsis viridis. Biochemical Genetics 1, 413–427.CrossRefGoogle Scholar
Baker, R. J., Honeycutt, R. L., Arnold, M. L., Sarich, V. M. & Genoways, H. H. (1981). Electrophoretic and immunological studies on the relationship of the Brachyphyllinae and the Glossophaginae. Journal of Mammalogy 62, 665–672.CrossRefGoogle Scholar
Barrowclough, G. F. (1980). Genetic and phenotypic differentiation in a wood warbler (Genus Dendroica) hybrid zone. Auk 97, 655–658.Google Scholar
Beck, M. L. & Price, J. O. (1981). Genetic variation in the terrestrial isopod, Armadillidium vulgare. Journal of Heredity 72, 15–18.CrossRefGoogle ScholarPubMed
Blanc, F. & Cariou, M.-L. (1980). High genetic variability of lizards of the sand-dwelling Lacertid genus Acanthodactylus. Genetica 54, 141–147.CrossRefGoogle Scholar
Brittnacher, J. G., Sims, S. R. & Ayala, F. J. (1978). Genetic differentiation between species of the genus Speyeria (Lepidoptera: Nymphalidae). Evolution 32, 199–210.CrossRefGoogle ScholarPubMed
Brown, K. (1981). Low genetic variability and high similarities in the crayfish genera Cambarus and Procambarus. American Midlands Naturalist 105, 225–232.CrossRefGoogle Scholar
Buroker, N. E., Hershberger, W. K. & Chew, K. K. (1979 a). Population genetics of the family Ostreidae. I. Intraspecific studies of Crassostrea gigas and Saccostrea commercialis. Marine Biology 54, 157–169.CrossRefGoogle Scholar
Buroker, N. E., Hershberger, W. K. & Chew, K. K. (1979 b). Population genetics of the family Ostreidae. II. Interspecific studies of the genera Crassostrea and Saccostrea. Marine Biology 54, 171–184.CrossRefGoogle Scholar
Buth, D. G. (1979). Biochemical systematics of the Cyprinid genus Notropis. 1. The subgenus Luxilus. Biochemical Systematics and Ecology 7, 69–79.CrossRefGoogle Scholar
Buth, D. G. (1980). Evolutionary genetics and systematic relationships in the catostomid genus Hypentelium. Copeia (1980), 280–290.CrossRefGoogle Scholar
Buth, D. G., Burr, B. M. & Schenck, J. R. (1980). Electrophoretic evidence for relationships and differentiation among members of the percid subgenus Microperca. Biochemical Systematics and Ecology 8, 297–304.CrossRefGoogle Scholar
Carlson, D. M., Kettler, M. K., Fisher, S. E. & Whitt, G. S. (1982). Low genetic variability in paddlefish populations. Copeia (1982), 721–725.CrossRefGoogle Scholar
Case, S. M. (1978). Electrophoretic variation in two species of Ranid frogs, Rana boylei and R. muscosa. Copeia (1978), 311–320.CrossRefGoogle Scholar
Chakraborty, R., Fuerst, P. A. & Nei, M. (1978). Statistical studies on protein polymorphism in natural populations. II. Gene differentiation between populations. Genetics 88, 367–390.CrossRefGoogle ScholarPubMed
Chakraborty, R., Fuerst, P. A. & Nei, M. (1980). Statistical studies on protein polymorphism in natural populations: III. Distribution of allele frequencies and the number of alleles per locus. Genetics 94, 1039–1063.CrossRefGoogle ScholarPubMed
Chakraborty, R. & Hedrick, P. W. (1983). Heterozygosity and genetic distance of proteins. Nature 304, 755.CrossRefGoogle ScholarPubMed
Chakraborty, R. & Nei, M. (1977). Bottleneck effects on average heterozygosity and genetic distance with the stepwise mutation model. Evolution 31, 347–356.CrossRefGoogle ScholarPubMed
Chambers, S. M. (1980). Genetic divergence between populations of Goniobasis (Pleuroceridae) occupying different drainage systems. Malacologia 20, 63–82.Google Scholar
Cianchi, R., Maini, S. & Bullini, L. (1980). Genetic distance between pheromone strains of the European corn borer, Ostrinia nubilalis: different contribution of variable substrate, regulatory and non-regulatory enzymes. Heredity 45, 383–388.CrossRefGoogle Scholar
Cianchi, R., Urbanelli, S., Coluzzi, M. & Bullini, L. (1978). Genetic distance between 2 sibling species of the Aedes mariae complex (Diptera, Culicidae). Parassitologia (Rome) 20, 39–46.Google ScholarPubMed
Cockley, D. E., Gooch, J. L. & Weston, D. P. (1977). Genie diversity in cave-dwelling crickets (Ceuthophilus gracilipes). Evolution 31, 313–318.Google Scholar
Daly, J. C., Wilkinson, P. & Shaw, D. D. (1981). Reproductive isolation in relation to allozymic and chromosomal differentiation in the grasshopper Coledla captiva. Evolution 35, 1164–1179.CrossRefGoogle ScholarPubMed
Davis, B. J., Demartini, E. E. & McGee, K. (1981). Gene flow among populations of a teleost (painted greenling, Oxylebius pictus) from Puget Sound to southern California, USA. Marine Biology 65, 17–24.CrossRefGoogle Scholar
Dew, R. D. & Kennedy, M. L. (1980). Genie variation in raccoons, Procyon lotor. Journal of Mammalogy 61, 697–702.CrossRefGoogle Scholar
Dillon, R. T. & Davis, G. M. (1980). The Goniobasis of southern Virgina and northwestern North Carolina, USA: genetic and shell morphometric relationships. Malacologia 20, 83–98.Google Scholar
Fairbairn, D. J. (1981 a). Biochemical genetic analysis of population differentiation in Greenland halibut (Reinhardtius hippoglossoides) from the Northwest Atlantic, Gulf of St Lawrence, and Bering Sea. Canadian Journal of Fisheries and Aquatic Science 38, 669–677.CrossRefGoogle Scholar
Fairbairn, D. J. (1981 b). Which witch is which ? A study of the stock structure of witch flounder (Glyptocephalus cynoglossus) in the Newfoundland region. Canadian Journal of Fisheries and Aquatic Science 38, 782–794.CrossRefGoogle Scholar
Feder, J. H. (1979). Natural hybridization and genetic divergence between the toads Bufo boreas and Bufo punctatus. Evolution 33, 1089–1097.CrossRefGoogle ScholarPubMed
Ferguson, M. M., Noakes, D. L. G. & Danzmann, R. G. (1981). Morphological and biochemical systematics of chubs, Nocomis biguttatus and N. micropogon (Pisces: Cyprinidae), in southern Ontario. Canadian Journal of Zoology 59, 771–775.CrossRefGoogle Scholar
Ferris, S. D., Buth, D. G. & Whitt, G. S. (1982). Substantial genetic differentiation among populations of Catostomus plebeius. Capeia (1982), 444–449.CrossRefGoogle Scholar
Fevolden, S. E. & Ayala, F. J. (1981). Enzyme polymorphism in Antarctic krill (Euphausi-acea); Genetic variation between populations and species. Sarsia 66, 167–182.CrossRefGoogle Scholar
Fuerst, P. A., Chakraborty, R. & Nei, M. (1977). Statistical studieson protein polymorphism in natural populations. I. Distribution of single locus heterozygosity. Genetics 86, 455–483.CrossRefGoogle Scholar
Galleguillos, R. A. & Ward, R. D. (1982). Genetic and morphological divergence between populations of the flatfish Platichthys flesus (L.) (Pleuronectidae). Biological Journal of the Linnean Society, 17, 395–408.CrossRefGoogle Scholar
Glover, D. G., Smith, M. H., Ames, L., Jule, J. & Dubach, J. M. (1977). Genetic variation in Pika populations. Canadian Journal of Zoology 55, 1841–1845.CrossRefGoogle Scholar
Gonzalez, A. M., Cabrera, V. M., Larruga, J. M. & Gullon, A. (1982). Genetic distance in the sibling species Drosophila melanogaster, Drosophila simulans and Drosophila mauritiana. Evolution 36, 517–522.CrossRefGoogle ScholarPubMed
Gorman, G. C., Buth, D. G., Soule, M. & Yang, S. Y. (1980). The relationships of the Anolis cristatellus species group: Electrophoretic analysis. Journal of Herpetology 14, 269–278.CrossRefGoogle Scholar
Gould, S. J. & Woodruff, D. S. (1978). Natural history of Cerion VIII. Little Bahama Bank – a revision based on genetics, morphometrics and geographic distribution. Bulletin of the Museum of Comparative Zoology, Harvard University, USA 148, 371–415.Google Scholar
Grant, W. S. & Utter, F. M. (1980). Biochemical genetic variation in walleye pollock Theragra chalcogramma: population structure in the south eastern Bering Sea and the Gulf of Alaska. Canadian Journal of Fisheries and Aquatic Science 37, 1093–1100.CrossRefGoogle Scholar
Guttman, S. I., Wood, T. K. & Karlin, A. A. (1981). Genetic differentiation along host plant lines in the sympatric Enchenopa binotata complex (Homoptera, Membracidae). Evolution 35, 205–217.CrossRefGoogle ScholarPubMed
Hafner, D. J., Petersen, K. E. & Yates, T. L. (1981). Evolutionary relationships of jumping mice (genus Zapus) of the south western USA. Journal of Mammalogy 62, 501–512.CrossRefGoogle Scholar
Harrison, R. G. (1979). Speciation in North American field crickets: evidence from electro-phoretic comparisons. Evolution 33, 1009–1023.CrossRefGoogle Scholar
Hedgecock, D. (1979). Biochemical genetic variation and evidence of speciation in Chthamalus barnacles of the tropical eastern Pacific Ocean. Marine Biology 54, 207–214.CrossRefGoogle Scholar
Hedgecock, D., Nelson, K., Simons, J. & Shleser, R. (1977). Genie similarity of American and European species of the lobster Homarus. Biological Bulletin 152, 41–50.CrossRefGoogle Scholar
Hertz, P. E. & Zouros, E. (1982). Genetic variability in two West Indian anoles (Reptilia, Iguanidae): relation to field thermal biology. Journal of Zoology 196, 499–518.CrossRefGoogle Scholar
Hillis, D. M. (1984). Misuse and modification of Nei's genetic distance. Systematic Zoology 33, 238–240.CrossRefGoogle Scholar
Hornbach, D. J., McLeod, M. J. & Guttman, S. I. (1980). On the validity of the genus Musculium (Bivalvia: Sphaeriidae): Electrophoretic evidence. Canadian Journal of Zoology 58, 1703–1707.CrossRefGoogle Scholar
Hornbach, D. J.McLeod, M. J., Guttman, S. I. & Seilkop, S. K. (1980). Genetic and morphological variation in the freshwater clam, Sphaerium (Bivalvia: Sphaeriidae). Journal of Molluscan Studies 46, 158–170.Google Scholar
Kawamoto, Y. & Ischak, T. M. (1981). Genetic differentiation of the Indonesian crab-eating macaque (Macaca fascicularius). 1. Preliminary report on blood protein polymorphism. Primates 22, 237–252.CrossRefGoogle Scholar
Kim, Y. J., Gorman, G. C.Papenfuss, T. & Roychoudhury, A. K. (1976). Genetic relationships and genetic variation in the Amphisbaenian genus Bipes. Copeia (1976), 120–124.CrossRefGoogle Scholar
Kimura, M. & Crow, J. R. (1964). The number of alleles that can be maintained in a finite population. Genetics 61, 763–771.CrossRefGoogle Scholar
Kluge, A. G. & Kerfoot, W. C. (1973). The predictability and regularity of character divergence. American Naturalist 107, 426–442.CrossRefGoogle Scholar
Kornfield, I., Beland, K. F., Moring, J. R. & Kircheis, F. W. (1981). Genetic similarity among endemic Arctic char (Salvelinus alpinus) and implications for their management. Canadian Journal of Fisheries and Aquatic Science 38, 32–39.CrossRefGoogle Scholar
Kovacic, D. A. & Guttman, S. I. (1979). Electrophoretic comparison of genetic variability between eastern and western populations of the opossum (Didelphis Virginiana). American Midlands Naturalist 101, 269–277.CrossRefGoogle Scholar
Krepp, S. R. & Smith, M. H. (1974). Genie heterozygosity in the 13-year cicada, Magicicada. Evolution 28, 396–401.CrossRefGoogle Scholar
Larson, A. (1980). Paedomorphosis in relation to rates of morphological and molecular evolution in the salamander Aneides flavipunctatus (Amphibia, Plethodontidae). Evolution 34, 1–17.CrossRefGoogle ScholarPubMed
Lawson, R. & Dessauer, H. C. (1979). Biochemical genetics and systematics of garter snakes of the Thamnophis elegans, Thamnophis couchii and Thamnophis ordinoides complex. Occasional Papers of the Museum of Zoology, Los Angeles State University, USA No. 56, 1–24.Google Scholar
Lewontin, R. C. (1974). The Genetic Basis of Evolutionary Change. New York: Columbia University Press.Google Scholar
Li, W. H. (1976). Electrophoretic identity of proteins in a finite population and genetic distance between taxa. Genetical Research 28, 119–127.CrossRefGoogle Scholar
Loudenslager, E. J. & Gall, G. A. E. (1980). Geographic patterns of protein variation and subspeciation in cutthroat trout, Salmo clarki. Systematic Zoology 29, 21–42.CrossRefGoogle Scholar
McKaye, A. R., Kocher, T., Reinthal, P. & Kornfield, I. (1982). A sympatric sibling species complex of Petrotilapia Trewavas from Lake Malawi analysed by enzyme electrophoresis (Pisces: Cichlidae). Zoological Journal of the Linnean Society 76, 91–96.CrossRefGoogle Scholar
McLeod, M. J., Wynes, D. L. & Guttman, S. I. (1980). Lack of biochemical evidence for hybridization between two species of darters. Comparative Biochemistry and Physiology, 67 B, 323–325.Google Scholar
Matthews, T. C. & Craig, G. B. (1980). Genetic heterozygosity in natural populations of the tree-hole mosquito Aedes triseriatus. Annals of the Entomological Society of America 73, 739–743.CrossRefGoogle Scholar
Mayer, W. (1981). Electrophoretic investigations on European species of the genera Lacerta and Podareis. III. Podareis tiliguerta, species or subspecies? Zoologischer Anzeiger 207, 151–157.Google Scholar
Mayer, W. & Tiedemann, F. (1981). Electrophoretic investigations on European species of the genus Lacerta and Podareis. II. To the systematic status of the lizards of the island Piperi (Northern Sporades, Greece). Zoologischer Anzeiger 207, 143–150.Google Scholar
Moran, C., Wilkinson, P. & Shaw, D. D. (1980). Allozyme variation across a narrow hybrid zone in the grasshopper, Caledia captiva. Heredity 44, 69–81.CrossRefGoogle Scholar
Morgan, R. P., Block, S. B., Ulanowicz, N. I. & Buys, C. (1978). Genetic variation in the soft-shelled clam, Mya arenaria. Estuaries 1, 255–258.Google Scholar
Mulley, J. C. & Latter, B. D. H. (1980). Genetic variation and evolutionary relationships within a group of 13 species of Penaeid prawns. Evolution 34, 904–916.CrossRefGoogle Scholar
Nemeth, S. T. & Tracey, M. L. (1979). Allozyme variability and relatedness in six crayfish species. Journal of Heredity 70, 37–43.CrossRefGoogle ScholarPubMed
Nei, M. (1972). Genetic distance between populations. American Naturalist 106, 283–292.CrossRefGoogle Scholar
Nei, M. (1975). Molecular Population Genetics and Evolution. Amsterdam, Oxford and New York: North-Holland.Google ScholarPubMed
Nei, M. (1978 a). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89, 583–590.CrossRefGoogle ScholarPubMed
Nei, M. (1978 b). The theory of genetic distance and evolution of human races. Japanese Journal of Human Genetics 23, 341–369.Google ScholarPubMed
Nei, M. (1983). Genetic polymorphism and the role of mutation in evolution. In Evolution of Genes and Proteins (ed. Nei, M. and Koehn, R. K.). Sunderland, Massachusetts: Sinauer.Google Scholar
Nei, M. & Chakraborty, R. (1973). Genetic distance and electrophoretic identity of proteins between taxa. Journal of Molecular Evolution 2, 323–328.CrossRefGoogle ScholarPubMed
Nei, M., Chakraborty, R. & Fuerst, P. A. (1981). Statistical studies of protein polymorphism - a reply. Genetics 97, 495.CrossRefGoogle Scholar
Nei, M. & Roychoudhury, A. K. (1974). Sampling variances of heterozygosity and genetic distance. Genetics 76, 379–390.CrossRefGoogle ScholarPubMed
Nevo, E. (1978). Genetic variation in natural populations: patterns and theory. Theoretical Population Biology 13, 121–177.CrossRefGoogle ScholarPubMed
Nevo, E. (1981). Genetic variation and climatic selection in the lizard Agama stellio in Israel and Sinai. Theoretical and Applied Genetics 60, 369–380.CrossRefGoogle ScholarPubMed
Nevo, E., Bar-El, C., Bar, Z. & Beiles, A. (1981). Genetic structure and climatic correlates of desert landsnails. Oecologia 48, 199–208.CrossRefGoogle ScholarPubMed
Nevo, E. & Yang, S. Y. (1979). Genetic diversity and climatic determinants of tree frogs in Israel. Oecologia 41, 47–63.CrossRefGoogle ScholarPubMed
Nicklas, N. L. & Hoffmann, R. J. (1979). Genetic similarity between two morphologically similar species of polychaetes. Marine Biology 52, 53–59.CrossRefGoogle Scholar
Ohta, T. & Kimura, M. (1973). A model of mutation appropriate to estimate the number of electrophoretically detectable alleles in a finite population. Genetical Research 22, 201–204.CrossRefGoogle Scholar
Patton, J. L., Sherwood, S. W. & Yang, S. Y. (1981). Biochemical systematics of chaetodipine pocket mice, genus Perognathus. Journal of Mammalogy 62, 477–492.CrossRefGoogle Scholar
Phillips, P. R. & Mayo, O. (1981). Statistical studies of protein polymorphism in natural populations. Genetics 97, 495.CrossRefGoogle ScholarPubMed
Pierce, B. A. & Mitton, J. B. (1979). A relationship of genetic variation within and among populations: An extension of the Kluge—Kerfoot phenomenon. Systematic Zoology 28, 63–70.CrossRefGoogle Scholar
Ramsey, P. R., Avise, J. C., Smith, M. H. & Urbston, D. F. (1979). Biochemical variation and genetic heterogeneity in South Carolina deer populations. Journal of Wildlife Management 43, 136–142.CrossRefGoogle Scholar
Rice, M. C., Gardner, M. B. & O'Brien, S. J. (1980). Genetic diversity in leukemia-prone feral house mice infected with murine leukemia virus. Biochemical Genetics 18, 915–928.CrossRefGoogle ScholarPubMed
Riska, B. (1979). Character variability and evolutionary rate in Menidia. Evolution 33, 1001–1004.CrossRefGoogle ScholarPubMed
Ritte, U. & Pashtan, A. (1982). Extreme levels of genetic variability in two Red Sea Cerithium species (Gastropoda: Cerithidae). Evolution 36, 403–407.CrossRefGoogle ScholarPubMed
Rohlf, F. J., Gilmartin, A. J. & Hart, G. (1983). The Kluge-Kerfoot phenomenon - a statistical artifact. Evolution 37, 180–202.Google ScholarPubMed
Ryman, N., Reuterwall, C., Nygren, K. & Nygren, T. (1980). Genetic variation and differentiation in Scandinavian moose (Alces alces): are large marnais monomorphic ? Evolution 34, 1037–1049.Google Scholar
Sage, R. D. & Selander, R. K. (1975). Trophic radiation through polymorphism in cichlid fishes. Proceedings of the National Academy of Sciences, USA 72, 4669–4673.CrossRefGoogle ScholarPubMed
Sattler, P. W. (1980). Genetic relationships among selected species of North American Scaphiopus. Capeia (1980), 605–610.CrossRefGoogle Scholar
Saura, A., Halkka, O. & Lokki, J. (1973). Enzyme gene heterozygosity in small island populations of Philaenus spumarius (L.) (Homoptera). Genetica 44, 459–473.CrossRefGoogle Scholar
Sbordoni, V., Allegrucci, G., Caccone, A., Cesaroni, D., Sbordoni, M. C. & Matthaeis, E. De (1981). Genetic variability and divergence in cave populations of Troglophilus cavicela and Troglophilus andreinii (Orethoptera, Rhaphidophoridae). Evolution 35, 226–233.CrossRefGoogle ScholarPubMed
Schmitt, L. H. (1978). Genetic variation in isolated populations of the Australian bush rat, Rattus fuscipes. Evolution 32, 1–14.CrossRefGoogle ScholarPubMed
Seidel, M. E., Reynolds, S. L. & Lucchino, R. V. (1981). Phylogenetic relationships among musk turtles (genus Sternotherus) and genie variation in Sternotherus odoratus. Herpetologica 37, 161–165.Google Scholar
Selander, R. K. (1976). Genie variation in natural populations. In Molecular Evolution (ed. Ayala, F. J.). Sunderland, Massachusetts: Sinauer.Google Scholar
Selander, R. K., Yang, S. Y., Lewontin, R. C. & Johnson, W. E. (1970). Genetic variation in the horseshoe crab (Limulus polyphemus), a phylogenetic ‘relie’. Evolution 24, 402–414.Google Scholar
Simonsen, V. (1982). Electrophoretic variation in large mammals. II. The red fox, Vulpes vulpes, the stoat, Mustela erminea, the weasel, Mustela putorius, the pine marten, Martes martes, the beech marten, Martes faina, and the badger, Meles meles. Hereditas 96, 299–305.CrossRefGoogle Scholar
Skibinski, D. O. F. & Ward, R. D. (1981). Relationship between allozyme heterozygosity and rates of divergence. Genetical Research 38, 71–92.CrossRefGoogle Scholar
Skibinski, D. O. F. & Ward, R. D. (1982). Correlations between heterozygosity and evolutionary rate of proteins. Nature 298, 490–492.CrossRefGoogle Scholar
Skibinski, D. O. F. & Ward, R. D. (1983). Heterozygosity and genetic distance of proteins - reply to Chakraborty & Hedrick. Nature 304, 755–756.CrossRefGoogle Scholar
Sluss, T. P., Sluss, E. S., Graham, H. M. & Dubois, M. (1978). Allozyme differences between Helothis virescens and H. zea. Annals of the Entomological Society of America 71, 191–195.CrossRefGoogle Scholar
Smith, M. F. (1979). Geographic variation in genie and morphological characters in Peromyscus californicus. Journal of Mammalogy 60, 705–722.CrossRefGoogle Scholar
Smith, M. F. & Patton, J. L. (1980). Relationships of pocket gophers (Thomomys bottae) populations of the lower Colorado River. Journal of Mammalogy 61, 681–696.CrossRefGoogle Scholar
Smith, P. J., Francis, R. I. C. C. & Paul, L. J. (1978). Genetic variation and population structure in the New Zealand snapper. New Zealand Journal of Marine and Freshwater Research 12, 343–350.CrossRefGoogle Scholar
Smith, P. J., McKoy, J. L. & Machin, P. J. (1980). Genetic variation in the rock lobsters Jasus edwardsii and Jasus novaehollandiae. New Zealand Journal of Marine and Freshwater Research 14, 55–63.CrossRefGoogle Scholar
Smith, P. J., Wood, B. A. & Benson, P. G. (1979). Electrophoretic and meristic separation of blue maomao and sweep. New Zealand Journal of Marine and Freshwater Research 13, 549–551.CrossRefGoogle Scholar
Sokal, R. R. (1976). The Kluge—Kerfoot phenomenon reexamined. American Naturalist 110, 1077–1091.CrossRefGoogle Scholar
Sokal, R. R. (1978). Population differentiation: something new or more of the same? In Ecological Genetics: the Interface (ed. Brussard, P. F.). New York: Springer-Verlag.Google Scholar
Steiner, W. W. M., Kitzmiller, J. B. & Osterbur, D. L. (1980). Gene differentiation in chromosome races of Anopheles nuneztovari. Mosquito Systematics 12, 306–319.Google Scholar
Stock, M. W. & Castrovillo, P. J. (1981). Genetic relationships among representative populations of five Choristoneura species: C. occidentalis, C. retiniana, C. biennis, C. lambertiana, and C.fumiferana (Lepidoptera: Tortricidae). Canadian Entomologist 113, 857–865.CrossRefGoogle Scholar
Stoneking, M., Wagner, D. J. & Hildebrand, A.C. (1981). Genetic evidence suggesting subspecific differences between northern and southern populations of brook trout (Salvelinus fontinalis). Copeia (1981), 810–819.CrossRefGoogle Scholar
Tilley, S. G. & Schwerdtfeger, P. M. (1981). Electrophoretic variation in Appalachian populations of the Desmognathus fuscus complex (Amphibia: Plethodontidae). Copeia (1981), 109–119.CrossRefGoogle Scholar
Tracey, M. L., Nelson, K., Hedgecock, D., Shleser, R. A. & Pressick, M. L. (1975). Biochemical genetics of lobsters: genetic variation and the structure of American lobster (Homarus americanus) populations. Journal of the Fisheries Research Board of Canada 32, 2091–2101.CrossRefGoogle Scholar
Vuorinen, J., Himberg, M. K-J. & Lankinen, P. (1981). Genetic differentiation in Coregonus albula (L.) (Salmonidae) populations in Finland. Hereditas 94, 113–122.CrossRefGoogle Scholar
Ward, R. D. & Warwick, T. (1980). Genetic differentiation in the molluscan species Littorina rudis and Littorina arcana (Prosobranchia: Littorinidae). Biological Journal of the Linnean Society 14, 417–428.CrossRefGoogle Scholar
Winans, G. A. (1980). Geographic variation in the milkfish, Chanos chanos. 1. Biochemical evidence. Evolution 34, 558–574.Google Scholar
Yanev, K. P. & Wake, D. B. (1981). Genie differentiation in a relict desert salamander, Batrachoseps campi. Herpetologica 37, 16–28.Google Scholar
Yang, S. Y. & Patton, J. L. (1981). Genie variability and differentiation in the Galapagos finches. Auk 98, 230–242.Google Scholar
Zera, A. J. (1981). Genetic structure of 2 species of water striders (Gerridae, Hemiptera) with differing degrees of winglessness. Evolution 35, 218–225.CrossRefGoogle Scholar