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Nucleolus organizer-suppressed position-effect variegation in Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

Janice B. Spofford  and
Rob DeSalle
Janice B. Spofford*
Affiliation:
Department of Ecology and Evolution, University of Chicago, 1103 E. 57th St, Chicago, Illinois 60637, USA
Rob DeSalle
Affiliation:
Department of Ecology and Evolution, University of Chicago, 1103 E. 57th St, Chicago, Illinois 60637, USA
*
* Corresponding author.
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The white locus is inactivated in a cell-by-cell variegated pattern when juxtaposed with the proximal or distal parts of the nucleolus organizer region (NO) by X chromosome inversion. Recombinants for two such inversions, wm51b and wm4, were obtained and randomized for genetic background. White locus activity was much higher in the wm4 recombinant duplicated for most of the NO and much lower in the wm51b recombinant deficient for it. Although there may be other molecular differences between the heterochromatic regions of the recombinants, the most obvious is the dosage of NO. Suppression of a NO region-evoked variegated phenotype by additional NO doses is discussed in relation to four different classes of models for position-effect variegation (PEV): chromatin structure, nuclear geometry, incomplete transposition of mobile elements, and heterochromatin promoter-driven transcription. A corollary of the structural model is functional subdivision of heterochromatin, which would enable the use of PEV as a tool for its study.

Type
Research Article
Information
Genetics Research , Volume 57 , Issue 3 , June 1991 , pp. 245 - 255
Copyright
Copyright © Cambridge University Press 1991

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