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Novel pleiotropic effect of rifampicin resistance mutation in a Micromonospora sp

Published online by Cambridge University Press:  14 April 2009

István Gadó ,
Csaba Kari ,
Valéria Széll  and
György Szvoboda
István Gadó
Affiliation:
Institute for Drug Research, Budapest, Hungary;
Csaba Kari
Affiliation:
Genetic Institute, Biological Center of the Hungarian Academy of Science at Szeged, Hungary
Valéria Széll
Affiliation:
Institute for Drug Research, Budapest, Hungary;
György Szvoboda
Affiliation:
Institute for Drug Research, Budapest, Hungary;
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Rifampicin-resistant mutants have been isolated from a Micromonospora sp. In one of these, rifampicin failed to inhibit [3H]UTP incorporation in osmotically shocked cells; consequently, resistance was probably not due to the alteration of rifampicin permeability. Parallel to the rifampicin resistance there was a substantial increase in the novobiocin sensitivity of the mutants. Rifampicin-sensitive revertants exhibited their original novobiocin sensitivity. At the same time there was no increase in their sensitivity towards coumermycin A1, an agent of related structure and activity. The possible mechanism for this pleiotropy is discussed.

Type
Research Article
Information
Genetics Research , Volume 40 , Issue 1 , August 1982 , pp. 33 - 40
Copyright
Copyright © Cambridge University Press 1982

References

REFERENCES

Bagdasarian, M. M., Izakowska, M. & Bagdasarian, M. (1977). Suppression of the dnaA phenotype by mutations in the rpoB cistron of ribonucleic acid polymerase in Salmonella typhimurium and Escherichia coli. Journal of Bacteriology 130, 577–582.CrossRefGoogle ScholarPubMed
Botchan, P., Wang, J. C. & Echols, H. (1973). Effect of circularity and superhelicity on transcription from bacteriophage λ DNA. Proceedings of the National Academy of Sciences, U.S.A. 70, 3077–3081.CrossRefGoogle Scholar
Doi, R. H., Brown, L. R., Rodgers, G. & Hsu, Y. (1970). Bacillus subtilis mutant altered in spore morphology and in RNA polymerase activity. Proceedings of the National Academy of Sciences, U.S.A. 66, 404–410.CrossRefGoogle ScholarPubMed
Gadó, I., Kari, Cs., Szvoboda, Gy. & Ambrus, G. (1979). A novel pleiotropic effect of the mutation of rifampicin resistance in Micromonospora. International Symposium on Antibiotics, 14th-18th May 1979 Weimar, GDR. Abstracts of papers C-9.Google Scholar
Gellert, M., O'Dea, M. H., Itoh, T. & Tomizawa, J.-I. (1976). Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase. Proceedings of the National Academy of Sciences, U.S.A. 73, 4474–4478.CrossRefGoogle ScholarPubMed
Grodzicker, T., Arditti, R. R. & Eisen, H. (1972). Establishment of repression by lambdoid phage in catabolite activator protein and adenylate cyclase mutants of Escherichia coli. Proceedings of the National Academy of Sciences, U.S.A. 69, 366–370.CrossRefGoogle ScholarPubMed
Gros, F., Gallant, J., Weisberg, R. & Cashel, M. (1967). Decryptification of RNA polymerase in whole cells of Escherichia coli. Journal of Molecular Biology 25, 555–557.CrossRefGoogle ScholarPubMed
Hong, J. S., Smith, G. R. & Ames, B. N. (1971). Adenosine 3′:5′-cyclic monophosphate concentration in the bacterial host regulates the viral decision between lysogeny and lysis. Proceedings of the National Academy of Sciences, U.S.A. 68, 2258–2262.CrossRefGoogle Scholar
Ikeuchi, T., Babasaki, K. & Kurahashi, K. (1979). Genetic evidence for possible interaction between a ribonucleic acid polymerase subunit and the spoOC gene product of Bacillus subtilis. Journal of Bacteriology 139, 327–332.CrossRefGoogle ScholarPubMed
Korch, C. T. & Doi, R. H. (1971). Electron microscopy of the altered spore morphology of a ribonucleic acid polymerase mutant of Bacillus subtilis. Journal of Bacteriology 105, 1110–1118.CrossRefGoogle ScholarPubMed
Leighton, T. J. (1973). An RNA polymerase mutation causing temperature-sensitive sporulation in Bacillus subtilis. Proceedings of the National Academy of Sciences, U.S.A. 70, 1179–1183.CrossRefGoogle ScholarPubMed
Michaeli, D., Molavi, A., Mirelman, D., Hanoch, A. & Weinstein, L. (1971). Mode of action of coumermycin A1: comparisons with novobiocin. Antimicrobial Agents and Chemotherapy 1970. 95–99.Google Scholar
Mirkin, S. M., Bogdanova, E. S., Gorlenko, Zh. M., Gragerov, A. I. & Larionov, O. A. (1979). DNA supercoiling and transcription in Escherichia coli: influence of RNA polymerase mutations. Molecular and General Genetics 117, 169–175.CrossRefGoogle Scholar
Oostra, B. A., Geert, A. B. & Gruber, M. (1970). Involvement of DNA gyrase in the transcription of ribosomal RNA. Nucleic Acid Research 8, 4235–4246.CrossRefGoogle Scholar
Ryan, M. J. (1976). Coumermycin A1: A preferential inhibitor of replicative DNA synthesis in Escherichia coli. I. In vivo characterization. Biochemistry 15, 3769–3777.CrossRefGoogle ScholarPubMed
Ryu, J.-I. (1978). Pleiotropic effect of rifampicin resistant mutation in Bacillus subtilis. Journal of Bacteriology 135, 408–414.CrossRefGoogle ScholarPubMed
Sanzey, B. (1979). Modulation of gene expression by drugs affecting deoxyribonucleic acid gyrase. Journal of Bacteriology 138, 40–47.CrossRefGoogle ScholarPubMed
Smith, D. H. & Davis, B. D. (1965). Inhibition of nucleic acid synthesis by novobiocin. Biochemical and Biophysical Research Communication 18, 796–800.CrossRefGoogle Scholar
Smith, C. L., Kubo, M. & Imamoto, F. (1978). Promoter-specific inhibition of transcription by antibiotics which act on DNA gyrase. Nature 275, 420–423.CrossRefGoogle ScholarPubMed
Snyder, L. R. (1972). An RNA polymerase mutant of Escherichia coli defective in the T4 viral transcription program. Virology 50, 396–403.CrossRefGoogle ScholarPubMed
Sonenshein, A. L., Cami, B., Brevet, J. & Cote, R. (1974). Isolation and characterization of rifampicin-resistant and streptolydigin-resistant mutants of Bacillus subtilis with altered sporulation properties. Journal of Bacteriology 120, 253–265.CrossRefGoogle Scholar
Sonenshein, A. L. & Losick, R. (1970). RNA polymerase mutants blocked in sporulation. Nature 227, 906–909.CrossRefGoogle ScholarPubMed
Sumida-Yasmumoto, C. & Doi, R. H. (1977). Bacillus subtilis ribonucleic acid polymerase mutants conditionally temperature sensitive at various stages of sporulation. Journal of Bacteriology 129, 433–444.CrossRefGoogle Scholar
Wang, J. C. (1974). Interactions between twisted DNAs and enzymes: the effects of superhelical turns. Journal of Molecular Biology 87, 797–816.CrossRefGoogle ScholarPubMed
Yang, H.-L., Heller, K., Gellert, M. & Zubay, G. (1979). Differential sensitivity of gene expression in vitro to inhibitors of DNA gyrase. Proceedings of the National Academy of Sciences, U.S.A. 76, 3304–3308.CrossRefGoogle ScholarPubMed