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New compound (1) chromosomes and the production of large quantities of X/O males in Drosophila hydei

Published online by Cambridge University Press:  14 April 2009

H. Beck
Affiliation:
Laboratoire de Génétique animale et végétale, R. de Malagnou 154, Université de Genève, Switzerland
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In view of the exceptional usefulness of Drosophila hydei for the analysis of Y-chromosome activity, a technique has been developed which permits the production of large numbers of males lacking a Y chromosome. It is based on the synthesis of new compound(1) chromosomes which carry sufficient rDNA genes to secure survival in the absence of any Y-chromosomal rDNA. Through non-disjunction in males of suitable stocks, fertile Compound(l) females which lack the Y chromosome, phenotypically distinguishable from their normal sibs, are produced in sufficient number to allow the subsequent breeding of X/O males in gram quantities for biochemical studies.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1975

References

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