Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-24T07:35:29.025Z Has data issue: false hasContentIssue false
  • English
  • Français

Mutants of bacteriophage lambda able to grow on the restricting host Escherichia coli strain W

Published online by Cambridge University Press:  14 April 2009

S. W. Glover  and
J. Aronovitch
S. W. Glover
Affiliation:
Medical Research Council, Microbial Genetics Research Unit, Hammersmith Hospital, Ducane Road, London, W.12
J. Aronovitch
Affiliation:
Medical Research Council, Microbial Genetics Research Unit, Hammersmith Hospital, Ducane Road, London, W.12
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Escherichia coli strain W adsorbs phage λ very efficiently but the phage does not form plaques on this strain because the DNA of λ is broken down in a majority of the infected cells shortly after adsorption. In a 10−3 to 10−4 fraction of the infected cells λ grows and small bursts of phage are produced. This phage does not carry the W-specific host modification and is unable to complete a second round of infection in W (Kerszman, Glover & Aronovitch, 1967). λω mutants have been isolated which are able to escape this restriction process and which plate on W with an efficiency of 1·0 and when grown in W these mutants carry a W-specific host modification.

Type
Short Papers
Information
Genetics Research , Volume 9 , Issue 1 , February 1967 , pp. 129 - 133
Copyright
Copyright © Cambridge University Press 1967

References

REFERENCES

Adams, M. H. (1950). Methods for the study of bacterial viruses. In Methods in Medical Research (Comroe, J. M., ed.), vol. 2, pp. 1–73. Chicago: The Year Book Publishers, Inc.Google Scholar
Appleyard, R. K. (1954). Segregation of new lysogenic types during growth of a doubly lysogenic strain derived from Escherichia coli K-12. Genetics, 39, 440–452.CrossRefGoogle Scholar
Arber, W. (1960). Polylysogeny for bacteriophage lambda. Virology, 11, 250–272.CrossRefGoogle Scholar
Arber, W. & Dussoix, D. (1962). Host specificity of DNA produced by Escherichia coli. I. Host controlled modification of bacteriophage λ. J. molec. Biol. 5, 18–36.CrossRefGoogle ScholarPubMed
Bertani, G. & Weigle, J. J. (1953). Host controlled variation in bacterial viruses. J. Bad. 65, 113–121.Google ScholarPubMed
Colson, C., Glover, S. W., Symonds, N. D. & Stacey, K. A. (1965). The location of the genes for host controlled modification and restriction in Escherichia coli K12. Genetics, 52, 1043–1050.CrossRefGoogle Scholar
Davis, B. D. (1950). Studies on nutritionally deficient bacterial mutants isolated by means of penicillin. Experientia, 6, 41–50.CrossRefGoogle Scholar
Glover, S. W. (1962). Valine resistant mutants of Escherichia coli K-12. Genet. Res. 3, 448–460.CrossRefGoogle Scholar
Glover, S. W. & Kerszman, G. (1967). The properties of a temperate bacteriophage Wø isolated from Escherichia coli strain W. Genet. Res. 9, 135–139.CrossRefGoogle Scholar
Jacob, F. & Wollman, E. L. (1954). Etude génétique d'un bactériophage tempéré d'Escherichia coli. I. Le système géné'tique du bactériophage λ. Annls Inst. Pasteur, Paris, 87, 653–673.Google Scholar
Kerszman, G., Glover, S. W. & Aronovitch, J. (1967). The restriction of phage λ in Escherichia coli strain W (in press).CrossRefGoogle Scholar
Weigle, J. J., Messelson, M. & Paigen, K. (1959). Density alterations associated with transducing ability in the bacteriophage lambda. J. molec. Biol. 1, 379–386.CrossRefGoogle Scholar