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Multi-step resistance to Chloramphenicol in RC-stringent Escherichia coli K12—its effect on the induction of RNA synthesis by antibiotics under amino acid starvation

Published online by Cambridge University Press:  14 April 2009

E. C. R. Reeve  and
J. O. Bishop
E. C. R. Reeve
Affiliation:
Institute of Animal Genetics, Edinburgh 9
J. O. Bishop
Affiliation:
Institute of Animal Genetics, Edinburgh 9
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A multi-step Chloramphenicol (CM)-resistant derivative of an RC-stringent strain of Escherichia coli auxotrophic for threonine and leucine was resistant also to Aureomycin (AM) and Puromycin (PM). All three antibiotics released the repression of RNA synthesis due to amino acid starvation in the CM-sensitive parent strain, their relative activities being about 1:10:100 for AM: CM: PM. High doses of AM and CM failed to induce RNA synthesis. The CM-resistant strain required greater concentrations of each antibiotic than the sensitive strain to induce the same level of RNA synthesis, and appeared to be about one hundred times, ten times and five times more resistant to CM, AM and PM, respectively, than the sensitive strain.

Type
Short Notes
Information
Genetics Research , Volume 6 , Issue 2 , July 1965 , pp. 304 - 309
Copyright
Copyright © Cambridge University Press 1965

References

REFERENCES

Adams, M. H. (1959). Bacteriophages. New York: Interscience Publishers.CrossRefGoogle Scholar
Aronson, A. I. & Spiegelman, S. (1958). On the use of chloramphenicol inhibited systems for investigating RNA protein synthesis. Biochim. biophys. Acta, 29, 214–215.CrossRefGoogle Scholar
Brock, T. D. (1961). Chloramphenicol. Bact. Rev. 25, 32–48.CrossRefGoogle ScholarPubMed
Cavalli, L. L. (1952). Genetic analysis of drug resistance. Bull. Wld Hlth Org. 6, 185–206.Google ScholarPubMed
Cavalli, L. L. & Maccacaro, G. A. (1952). Polygenic inheritance of drug-resistance in the bacterium Escherichia coli. Heredity, Lond. 6, 311–331.CrossRefGoogle Scholar
Franklin, T. J. (1963). The inhibition of incorporation of leucine into protein of cell-free systems of rat liver and Escherichia coli by chlortetracycline. Biochem. J. 87, 449–453.CrossRefGoogle ScholarPubMed
Goldberg, I. H. & Reich, E. (1964). Actinomycin inhibition of RNA synthesis directed by DNA. Fedn Proc. 23, 958–964.Google ScholarPubMed
Heywood, A. M. & Sinsheimer, R. C. (1963). Inhibition of protein synthesis in E. coli protoplasts by actinomycin-D. J. molec. Biol. 6, 247–249.CrossRefGoogle Scholar
Kurland, C. G. & Maaløe, O. (1962). Regulation of ribosomal and transfer RNA synthesis. J. molec. Biol. 4, 193–210.CrossRefGoogle ScholarPubMed
Nathans, D. V., Ehrenstein, G., Monro, R. & Lipmann, F. (1962). Protein synthesis from aminoacyl-soluble ribonucleic acid. Fedn Proc. 21, 127–133.Google ScholarPubMed
Reeve, E. C. R. & Bishop, J. O. (1965). Variations in resistance to three antibiotics among some single-step mutants to Chloramphenicol resistance in a strain of Escherichia coli K12. Genet. Res. 6, 310–315.CrossRefGoogle Scholar
Stent, G. S. & Brenner, S. (1961). A genetic locus for the regulation of ribonucleic acid synthesis. Proc. natn Acad. Sci. U.S.A. 47, 2005–2014.CrossRefGoogle ScholarPubMed
Taylor, A. L. & Adelberg, E. A. (1960). Linkage analysis with very high frequency males of Escherichia coli. Genetics, Princeton, 45, 1233–1243.CrossRefGoogle ScholarPubMed
Watanabe, T. (1963). Infective heredity of multiple drug resistance in bacteria. Bact. Rev. 27, 87–115.CrossRefGoogle ScholarPubMed
Yokota, T. & Akiba, T. (1961). Studies on the mechanism of transfer of drug-resistance in bacteria. VI (In Japanese). Med. Biol. (Tokyo), 58, 172–175.Google Scholar