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The molecular basis of instability of the singedvery weak mutation in Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

C. A. Ortori
Affiliation:
Department of Genetics, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, UK
D. Chambers
Affiliation:
Department of Genetics, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, UK
J. F. Y. Brookfield*
Affiliation:
Department of Genetics, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, UK
*
Corresponding author.
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Summary

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The singedvery weak mutation was created by the sequential addition of two P transposable elements to the singed gene. The mutation can be somatically unstable through the action of a dominant maternal effect mutation on the second chromosome. It is also unstable in the germ line in these conditions. Sequencing of the region of the P insertions in the mutation reveals that the two inserted elements have single internal deletions, and the larger of the two is a copy of the KP element. The mutation will generate, at high frequencies, strongly singed and pseudo-wild type products by reversions occurred in the germline. These are the result of the precise excision of the smaller and the larger elements respectively. By PCR amplification of dissected thoraces we show that the somatic instability of the mutation, from a weak to a strong singed phenotype, is also caused by the excision of the smaller of the two elements.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1994

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