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Mitotic mapping of Schizosaccharomyces pombe*

Published online by Cambridge University Press:  14 April 2009

Miguel Flores da Cunha
Miguel Flores da Cunha
Affiliation:
Division of BiologyThe University of Texas at Dallas‡Dallas, Texas 75230, U.S.A.
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Genetic mapping by means of mitotic haploidization (induced by parafluoropkenylalanine) and mitotic crossing-over was carried out with the fission yeast Schizosaccharomyces pombe. Thirty-two different genetic markers were involved in this investigation; some meiotic linkage relationships had been previously reported (Leupold, Megnet) for 16 of these loci. Mitotic haploidization experiments resulted in the genetic identification of six chromosomes in the haploid complement.

Furthermore, in an attempt to study the mechanism of action of parafluorophenylalanine (pFPA) on mitotic haploidization, pedigree analyses were performed by micromanipulation of diploid cells growing in the presence of pFPA. Haploid cells were detected after 40 hours of contact with the analogue and many lethal pedigree branches were observed. These observations seem to agree with Käfer's (1961) and Lhoa's (1968) suggestion that mitotic haploidization in Fungi is achieved by progressive loss of chromosomes throughout cell divisions.

Type
Research Article
Information
Genetics Research , Volume 16 , Issue 2 , October 1970 , pp. 127 - 144
Copyright
Copyright © Cambridge University Press 1970

References

REFERENCES

Angehrn, P. (1964). Untersuchungen über intragene Rekombinationsmechanismen und allele Komplementierung an Adeninmutanten von Schizosaccharomyces pombe. Ph.D. Thesis, University of Zürich, Switzerland.Google Scholar
Brock, T. D. (1961). Physiology of conjugation in the yeast Hansenula wingei. Journal of Microbiology 26, 487–497.Google Scholar
Emeis, C. (1966). Haploidisierung von diploiden Hefen durch p-Flurophenylalanin. Zeitschrift Naturforschung 21 b, 816–817.Google Scholar
Fincham, J. R. S. (1966). Genetic Complementation. New York: W. A. Benjamin.Google Scholar
Flores Da Cunha, M. (1969). Parassexualidade em Schizosaccharomyces pombe. Ph.D. Thesis, Federal University of Rio de Janeiro, Brazil.Google Scholar
Forbes, E. (1959). Use of mitotic segregation for assigning genes to linkage groups in Aspergillus nidulans. Heredity 13, 67–80.Google Scholar
Green, M. C. (1963). Methods for testing linkage. In Methodology in Mammalian Genetics, Burdette, W. J. (ed.), pp. 65–82. San Francisco: Holden-Day, Inc.Google Scholar
Gutz, H. (1963). Untersuchungen zur Feinstruktur der Gene ad7 und ad6 von Schizosaccharomyces pombe Lind. Habilitation Thesis, Technical University of Berlin, Germany.Google Scholar
Gutz, H. (1966). Induction of mitotic segregation with p-fluorophenylalanine in Schizosaccharomyces pombe. Journal of Bacteriology 92, 1567–1568.Google Scholar
Gutz, H. (1967 a). Twin meiosis and other ambivalences in the life cycle of Schizosaccharomyces pombe. Science 158, 796, 798.Google Scholar
Gutz, H. (1967 b). Zwillingsmeiose—eine neue Beobachtung bei einer Hefe. Bericht deutsch botanical Gesellschaft 80, 555–558.Google Scholar
Haefner, K. (1967 a). A simple apparatus for producing agar layers of uniform thickness for microbiological manipulator work. Zeitschrift Allegemeine Mikrobiologie 7, 229–231.Google Scholar
Haefner, K. (1967 b). A remark to the origin of pure mutant clones observed after treatment of Schizosaccharomyces pombe. Mutation Research 4, 514–516.Google Scholar
Halvorson, H. O. & Spiegelman, S. (1952). The inhibition of enzyme formation by amino acid analogues. Journal of Bacteriology 64, 207–221.Google Scholar
Heslot, H. (1960). Schizosaccharomyces pombe: un nouvel organisme pour l'étude de la mutagenèse chimique. Abhandlungen deutsch Akademie Wissenschaften Berlin (Klasse f. Medizin), 1960, 98–115.Google Scholar
Käfer, E. (1958). An 8-chromosome map of Aspergillus nidulans. Advances in Genetics 9, 105–145.Google Scholar
Käfer, E. (1961). The process of spontaneous recombination in vegetative nuclei of Aspergillus nidulans. Genetics 46, 1581–1609.Google Scholar
Kerridge, D. (1960). The effect of inhibitors on the formation of flagella by Salmonella typhimurium. Journal of General Microbiology 33, 519–538.Google Scholar
Lederberg, J. & Lederberg, E. M. (1952). Replica plating and indirect selection of Bacterial mutants. Journal of Bacteriology 63, 399–406.Google Scholar
Leupold, U. (1950). Die Vererbung von Homothallie und Heterothallie bei Schizosaccharomyces pombe. Compte rendu Travaux Laboratory Carlsberg (Ser. Physiol.) 24, 381–480.Google Scholar
Leupold, U. (1955 a). Versuche zur genetischen Klassifizierung adenin-abhängiger Mutanten von Schizosaccharomyces pombe. Archiv Julius Klaus-Stiftung Vererbungsforschung, Sozialanthropologie Rassenhygiene 30, 506–516.Google Scholar
Leupold, U. (1955 b). Methodisches zur Genetik von Schizosaccharomyces pombe. Schweizeizerische Zeitschrift allgemeine Pathologie Bakteriologie 18, 1141–1146.Google Scholar
Leupold, U. (1957). Physiologisch-genetische Studien an adenin-abhängigen Mutanten von Schizosaccharomyces pombe. Ein Beitrag zum Problem der Pseudoallelie. Schweizerische Zeitschrift allegemeine Pathologie Bakteriologie 20, 535–544.Google Scholar
Leupold, U. (1958). Studies on recombination in Schizosaccharomyces pombe. Cold Spring Harbor Symposia on quantitative Biology 23, 161–170.Google Scholar
Leupold, U. (1961). Intragene Rekombination und aliele Komplementierung. Archiv Julius Klaus-Stiftung Vererbungsforschung, Sozialanthropologie Rossenhygiene 36, 89–117.Google Scholar
Leupold, U. & Gutz, H. (1964). Genetic fine structure in Schizosaccharomyces. Proceedings XIth International Congress Genetics 2, 31–35.Google Scholar
Lhoas, P. (1961). Mitotic haploidization by treatment of Aspergillus niger diploids with para-fluorophenylalanine. Nature, London 190, 744.Google Scholar
Lhoas, P. (1967). Genetic analysis by means of the parasexual cycle in Aspergillus niger. Genetical Research 10, 45–61.Google Scholar
Lhoas, P. (1968). Growth rate and haploidization of Aspergillus niger on medium containing p-fluorophenylalanine. Genetical Research 12, 305–315.Google Scholar
Martin, G. J. & Moss, J. N. (1949). In vitro effects of metabolite dispiacere on Pseudomonas aeruginosa. American Journal of Pharmacy 121, 169–172.Google Scholar
McCully, K. & Forbes, E. (1965). The use of p-fluorophenylalanine with ‘master-strains’ of Aspergillus nidulans for assigning genes to linkage groups. Genetical Research 6, 352–359.Google Scholar
Morpurgo, G. (1961). Somatic segregation induced by p-fluorophenylalanine. Aspergillus News Letter 2, 10.Google Scholar
Mueller, G. C. & Kajiwara, K. (1966). Actinomycin D and p-fluorophenylalanine, inhibitors of nuclear replication in HeLa cells. Biochimica biophysica acta 119, 557–565.Google Scholar
Munier, R. & Cohen, G. N. (1959). Incorporation d'analogues structuraux d'aminoacides dans les protéines bactériennes au cours de leur synthèse in vivo. Biochimica biophysica acta 31, 378–391.Google Scholar
Pontecorvo, G. (1958). Trends in Genetic Analysis. New York: Columbia University Press.Google Scholar
Pontecorvo, G. & Käfer, E. (1958). Genetic analysis based on mitotic recombination. Advances in Genetics 9, 71–104.Google Scholar
Pontecorvo, G. & Roper, J. A. (1952). Genetic analysis without sexual reproduction by means of polyploidy in Aspergillua nidulans. Journal of General Microbiology 6, VII.Google Scholar
Pontecorvo, G. & Roper, J. A. (1953). Diploide and mitotic recombination. (In The Genetics of Aspergillus nidulans). Advances in Genetics 5, 218–238.Google Scholar
Pontecorvo, G., Tarr Gloor, E. & Forbes, E. (1954). Analysis of mitotic recombination in Aspergillus nidulans. Journal of Genetics 52, 226–237.Google Scholar
Rasmussen, L. & Zeuthen, E. (1962). Cell division and protein synthesis in Tetrahymena, as studied with p-fluorophenylalanine. Compte rendu Travaux Laboratoire Carlsberg 32, 333–358.Google Scholar
Roman, H. & Jacob, F. (1958). A comparison of spontaneous and ultraviolet-induced allelic recombination with reference to the recombination of outside markers. Cold Spring Harbor Symposia on Quantitative Biology 23, 155–160.CrossRefGoogle Scholar
Roper, J. A. (1966). Mechanisms of inheritance. 3. The parasexual cycle. In The Fungi, vol. II. Ainsworth, G. C. & Sussman, A. S. (ed.), pp. 589–617. New York: Academic Press.Google Scholar
Schopfer, W. H., Wustenfeld, D. & Turian, G. (1963). La division nucléaire chez Schizosaccharomyces pombe. Archiv Mikrobiologie 45, 304–313.Google Scholar
Sisken, J. E. & Wilkes, E. (1967). The time of synthesis and the conservation of mitosisrelated proteins in cultured human amnion cells. Journal of Cell Biology 34, 97–110.Google Scholar
Stern, C. (1936). Somatic crossing-over and segregation in Drosophila melanogaster. Genetics 21, 625–730.Google Scholar
Strömnaes, Ö. (1968). Genetic changes in Saccharomyces cerevisiae grown on media containing DL-para-fluorophenylalanine. Hereditas 59, 197–220.Google Scholar
Taylor, E. W. (1959). Dynamics of spindle formation and its inhibition by chemicals. Journal of biophysical and biochemical Cytology 6, 193–196.Google Scholar
Theichler, H. J. (1964). Genetische Feinstruktur und intragene Rekombinations-mechanismen im ad2-Locus von Schizosaccharomyces pombe. Ph.D. Thesis, University of Zürich, Switzerland.Google Scholar