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Mitochondrial instability in a strain of Saccharomyces cerevisiae

Published online by Cambridge University Press:  14 April 2009

I. H. Evans  and
D. Wilkie
I. H. Evans
Affiliation:
Department of Botany and Microbiology, University College London, London WC1E 6BT
D. Wilkie
Affiliation:
Department of Botany and Microbiology, University College London, London WC1E 6BT
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Summary

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A haploid strain of Saccharomyces cerevisiae has been described which, on glucose medium, segregates vegetatively a high frequency of mutants with different degrees of respiratory impairment. The range of mutants seemingly encompasses both non-revertible ρ- petites and revertible point mutations resembling leaky mit- mutations. The segregants have aberrant cytochrome contents and reduced growth capabilities on fermentable sugars other than glucose; these defects apparently correlate with the degree of respiratory impairment. Genetic analysis of this mutator phenomenon has implicated a nuclear gene which appears to show specificity of interaction with the mitochondrial genome as well as a requirement for glucose repression. The mutator effect seems to extend also to the loci in mitochondrial DNA for resistance to the antibiotics erythromycin and oligomycin.

Type
Research Article
Information
Genetics Research , Volume 32 , Issue 2 , October 1978 , pp. 171 - 182
Copyright
Copyright © Cambridge University Press 1978

References

REFERENCES

Bandlow, W. (1976). On the formation of rho-petites in yeast: VI. Expression of a mitochondrial conditional mutation controlling petite formation in Saccharomyces cerevisiae. In Genetics, Biogenesis and Bioenergetics of Mitochondria (ed. Bandlow, W., Schweyen, R. J., Thomas, D. Y., Wolf, K. and Kaudewitz, F.), pp. 179–202. Berlin and New York: Walter de Gruyter.CrossRefGoogle Scholar
Ephrussi, B. & Hottinguer, H. (1951). On an unstable state in yeast. Cold Spring Harbor Symposia on Quantitative Biology 16, 75–85.CrossRefGoogle Scholar
Evans, I. H. & Wilkie, D. (1976). Mitochondrial factors in the utilization of sugars in Saccharomyces cerevisiae. Genetical Research 27, 89–93.CrossRefGoogle ScholarPubMed
Flury, V., Mahler, H. R. & Feldman, F. (1974). A novel respiration-deficient mutant of Saccharomyces cerevisiae. Preliminary characterization of phenotype and mitochondrial inheritance of phenotype and mitochondrial inheritance. Journal of Biological Chemistry 249, 6130–6137.CrossRefGoogle ScholarPubMed
Linstead, D., Evans, I. H. & Wilkie, D. (1974). Mitochondrial genetic factors in the cellular response to chlorimipramine in Saccharomyces cerevisiae. In the Biogenesis of Mitochondria (ed. Kroon, A. M. and Saccone, C.), pp. 179–192. New York: Academic Press.CrossRefGoogle Scholar
Mahler, H. R. & Wilkie, D. (1978). Mitochondrial control of sugar utilization in Saccharomyces cerevisiae. Plasmid 1, 125–133.CrossRefGoogle ScholarPubMed
Nagai, S. (1962). Stabilizing effect of caffeine on a respirationally unstable strain of yeast. Experimental Cell Research 26, 253–259.CrossRefGoogle ScholarPubMed
Nagai, S., Yanagishima, N. & Nagai, H. (1961). Advances in the study of respirationdeficient (RD) mutation in yeast and other microrganisms. Bacteriological Reviews 25, 404–426.CrossRefGoogle Scholar
Putrement, A., Baranowska, H. & Prazmo, W. (1973). Induction of manganese of mitochondrial antibiotic resistant mutations in yeast. Molecular and General Genetics 126, 357–366.CrossRefGoogle Scholar
Rhoades, M. M. (1946). Plastid mutations. Cold Spring Harbor Symposia on Quantitative Biology II, 202–207.Google Scholar
Sherman, F. (1963). Respiration-deficient mutants of yeast. I. Genetics. Genetics 48, 375–385.CrossRefGoogle ScholarPubMed
Thomas, D. Y. & Wilkie, D. (1968). Inhibition of mitochondrial synthesis in yeast by erythromycin: cytoplasmic and nuclear factors controlling resistance. Genetical Research 11, 33–41.CrossRefGoogle ScholarPubMed
Timpson, J. (1977). Caffeine. Mutation Research 47, 1–52.CrossRefGoogle Scholar
Tsuboi, M. & Yakagishima, N. (1973). Effect of cyclic AMP, theophylline and caffeine on the glucose repression of sporulation in Saccharomyces cerevisiae. Archiv fur Mikrobiologie 93, 1–12.Google Scholar
Tzagaloff, A., Akai, A. & Needleman, R. B. (1975). Assembly of the mitochondrial membrane system: isolation of nuclear and cytoplasmic mutants of Saccharomyces cerevisiae, with specific defects in mitochondrial functions. Journal of Bacteriology 122, 826–831.CrossRefGoogle Scholar
Wilkie, D. (1972). The yeast cell in the antimitochondrial activity of drugs. Medical and Biological Illustrations 22, 119–124.Google ScholarPubMed
Wilkie, D. & Negrotti, T. (1968). Induction of cytoplasmic respiratory deficiency by repression of the respiratory system in a mutant of Saccharomyces cerevisiae. Biochimica Biophysica Acta 153, 341–349.Google Scholar