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Methods for the isolation and analysis of plasmodial mutants in Physarum polycephalum

Published online by Cambridge University Press:  14 April 2009

D. J. Cooke  and
Jennifer Dee
D. J. Cooke
Affiliation:
Department of Genetics, University of Leicester, Leicester LEI 7RH, England
Jennifer Dee
Affiliation:
Department of Genetics, University of Leicester, Leicester LEI 7RH, England
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Summary

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Amoebae of the Colonia isolate of Physarum polycephalum produce plasmodia within individual amoebal clones.

This paper reports the derivation from the Colonia strain C50 of a strain CL (Colonia Leicester) which produces plasmodia in clones with high efficiency and which completes the life cycle reliably and repeatedly in single clones. The derivation of a line CLd (CL delayed plasmodium formation) is described and, using CLd, the construction of the isogenic heterothallic strains mt1; CL and mt2; CL.

The above strains provide a system for the isolation and genetic analysis of mutants of P. polycephalum within a uniform genetic background, in particular mutants expressed in the plasmodium.

Using this system two auxotrophic mutants have been isolated. Preliminary genetic analysis has shown that they are due to single gene mutations.

Type
Research Article
Information
Genetics Research , Volume 24 , Issue 2 , October 1974 , pp. 175 - 187
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

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