Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-12-01T01:52:25.806Z Has data issue: false hasContentIssue false
  • English
  • Français

A method for selecting streptomycin-sensitive mutants of R factors which adenylate streptomycin*

Published online by Cambridge University Press:  14 April 2009

J. H. Harwood ,
J. Janjigian  and
D. H. Smith
J. H. Harwood
Affiliation:
Departments of Bacteriology and Immunology and Pediatrics, Harvard Medical School, and Children's Hospital Medical Center, Boston, Mass. 02115, U.S.A.
J. Janjigian
Affiliation:
Departments of Bacteriology and Immunology and Pediatrics, Harvard Medical School, and Children's Hospital Medical Center, Boston, Mass. 02115, U.S.A.
D. H. Smith
Affiliation:
Departments of Bacteriology and Immunology and Pediatrics, Harvard Medical School, and Children's Hospital Medical Center, Boston, Mass. 02115, U.S.A.
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

An Arg− conditionally streptomycin (Sm)-dependent strain of E. coli B that carries an R factor mediating Sm adenylation cannot grow on minimal agar containing 20 μg Sm/ml unless the strain either becomes Arg+ or loses the ability to adenylate Sm (Harwood & Smith, 1969b). We have therefore studied the efficacy of this selection with respect to enriching for SmS mutants of such R factors. Following u.v. mutagenesis, cells were plated on the selective medium; of the clones which were examined, Arg− R-SmS colonies were found up to 3000 times more frequently than Arg+ R-SmR colonies.

Type
Short Papers
Information
Genetics Research , Volume 14 , Issue 3 , December 1969 , pp. 323 - 327
Copyright
Copyright © Cambridge University Press 1969

References

REFERENCES

Davis, B. D. (1948). Isolation of biochemically deficient mutants of bacteria by penicillin. J. Am. Chem. Soc. 70, 4267.CrossRefGoogle ScholarPubMed
Davis, B. D. & Mingioli, E. S. (1950). Mutants of Escherichia coli requiring methionine or vitamin B12. J. Bact. 60, 17.CrossRefGoogle ScholarPubMed
Gorini, L. & Kataja, E. (1964). Phenotypic repair by streptomycin of defective genotypes in E. coli. Proc. natn. Acad. Sci. U.S.A. 51, 487.CrossRefGoogle ScholarPubMed
Harwood, J. H. & Smith, D. H. (1969 a). Resistance factor-mediated streptomycin resistance. J. Bact. 97, 1262.CrossRefGoogle ScholarPubMed
Harwood, J. H. & Smith, D. H. (1969 b). Phenotypic suppression by streptomycin-adenylate and bluensomycin-adenylate. Genet. Res., Camb. 14, 259–273.CrossRefGoogle ScholarPubMed
Jacob, F. & Wollman, E. L. (1955). Étude génétique d'un bactériophage tempéré d'Escherichia coli: Effet du rayonnement ultraviolet sur la recombinaison génétique. Ann. inst. Pasteur 88, 724.Google Scholar
Kondo, S., Okanishi, M., Utahara, R., Maeda, K. & Umezawa, H. (1968). Isolation of kanamycin and paromamine inactivated by E. coli carrying R factor. J. Antibiot. (Tokyo), A 21 (1), 22.CrossRefGoogle ScholarPubMed
Smith, D. H. (1969). R factor-mediated resistance to aminoglycoside antibiotics. J. Infect. Dis. 119, 378CrossRefGoogle Scholar
Walton, J. R. & Smith, D. H. (1969). New hemolysin (γ) produced by Escherichia coli. J. Bact. 98, 304.CrossRefGoogle ScholarPubMed
Watanabe, T. (1964). Selected methods of genetic study of episome-mediated drug resistance in bacteria. In Meth. med. Res. 10, 202.Google ScholarPubMed
Watanabe, T. & Fukasawa, T. (1961). Episome-mediated transfer of drug resistance in Enterobacteriaceae. III. Transduction of resistance factors. J. Bact. 82, 202.CrossRefGoogle ScholarPubMed
Watanabe, T. & Lyang, K. W. (1962). Episome-mediated transfer of drug resistance in Enterobacteriaceae. V. Spontaneous segregation and recombination of resistance factors in Salmonella typhimurium. J. Bact. 84, 422.CrossRefGoogle ScholarPubMed