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Mapping candidate genes for Drosophila melanogaster resistance to the parasitoid wasp Leptopilina boulardi

Published online by Cambridge University Press:  27 November 2006

M. HITA ,
E. ESPAGNE ,
F. LEMEUNIER ,
L. PASCUAL ,
Y. CARTON ,
G. PERIQUET  and
M. POIRIE
M. HITA
Affiliation:
Institut de Recherche sur la Biologie de l’Insecte – UMR CNRS 6035, Université François Rabelais, Parc Grandmont, 37200 Tours, France
E. ESPAGNE
Affiliation:
Institut de Recherche sur la Biologie de l’Insecte – UMR CNRS 6035, Université François Rabelais, Parc Grandmont, 37200 Tours, France Present address: Institut de Génétique et Microbiologie, Université Paris Sud, Bat. 400, 91405 Orsay cedex, France.
F. LEMEUNIER
Affiliation:
Laboratoire Populations, Génétique, Evolution CNRS, 91198 Gif sur Yvette, France
L. PASCUAL
Affiliation:
Departamento de Genetica, Universidad de Valencia, 46100 Burjassot, Valencia, Spain
Y. CARTON
Affiliation:
Laboratoire Populations, Génétique, Evolution CNRS, 91198 Gif sur Yvette, France
G. PERIQUET
Affiliation:
Institut de Recherche sur la Biologie de l’Insecte – UMR CNRS 6035, Université François Rabelais, Parc Grandmont, 37200 Tours, France
M. POIRIE
Affiliation:
Institut de Recherche sur la Biologie de l’Insecte – UMR CNRS 6035, Université François Rabelais, Parc Grandmont, 37200 Tours, France Present address: UMR 1112 UNSA INRA ‘Réaction des Organismes aux Stress Environnementaux’, 400 route des Chappes – BP 167, 06903 Sophia-Antipolis, France.
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Abstract

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Drosophila melanogaster resistance against the parasitoid wasp Leptopilina boulardi is under the control of a single gene (Rlb), with two alleles, the resistant one being dominant. Using strains bearing deletions, we previously demonstrated that the 55E2–E6; 55F3 region on chromosome 2R is involved in the resistance phenomenon. In this paper, we first restricted the Rlb containing region by mapping at the molecular level the breakpoints of the Df(2R)Pc66, Df(2R)P34 and Df(2R)Pc4 deficiencies, using both chromosomal in situ hybridization and Southern analyses. The resistance gene was localized in a 100 kb fragment, predicted to contain about 10 different genes. Male recombination genetic experiments were then performed, leading to identification of two possible candidates for the Rlb gene. Potential involvement of one of this genes, edl/mae, is discussed.

Type
Research Article
Information
Genetics Research , Volume 88 , Issue 2 , October 2006 , pp. 81 - 91
Copyright
© 2006 Cambridge University Press