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Mapping and biochemical analysis of Hor 4 (Hrd G), a second locus encoding B hordein seed proteins in barley (Hordeum vulgare L.)

Published online by Cambridge University Press:  14 April 2009

Peter R. Shewry*
Affiliation:
AFRC Institute of Arable Crops Research, Department of Biochemistry, Rothamsted Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
Saroj Parmar
Affiliation:
AFRC Institute of Arable Crops Research, Department of Biochemistry, Rothamsted Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
Julian Franklin
Affiliation:
AFRC Institute of Arable Crops Research, Department of Biochemistry, Rothamsted Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
Rodger White
Affiliation:
Department of Statistics, Rothamsted Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
*
* Corresponding author
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Summary

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The hordein polypeptide controlled by the Hrd G (Hor 4) locus in Elgina and derived lines was purified by preparative isoelectric focusing. The amino acid composition was similar to those of the major B hordeins encoded by the Hor 2 locus. Genetic analysis confirmed that Hor 4 is located proximally to Hor 1 on the short arm of chromosome 5. It is speculated that Hor 4 arose by translocation of genes from Hor 2, possibly in an ancestor of Elgina.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1988

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