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Map-based cloning of quantitative trait loci: progress and prospects

Published online by Cambridge University Press:  01 February 2002

D. L. REMINGTON ,
M. C. UNGERER  and
M. D. PURUGGANAN
D. L. REMINGTON
Affiliation:
Department of Genetics, North Carolina State University, Raleigh, NC 27695-7614
M. C. UNGERER
Affiliation:
Department of Genetics, North Carolina State University, Raleigh, NC 27695-7614
M. D. PURUGGANAN
Affiliation:
Department of Genetics, North Carolina State University, Raleigh, NC 27695-7614
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Abstract

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Map-based cloning has been considered problematic for isolating quantitative trait loci (QTLs) due to the confounding phenotypic effects of environment and other QTLs. However, five recent studies, all in plants, have succeeded in cloning QTLs using map-based methods. We review the important features of these studies and evaluate the prospects for broader application of the techniques. Successful map-based cloning requires that QTLs represent single genes that can be isolated in near-isogenic lines, and that genotypes can be unambiguously inferred by progeny testing. In plants or animals for which map-based cloning of genes with discrete phenotypes is feasible, the modified procedures required for QTLs should not be limiting in most cases. The choice between map-based cloning and alternative methods will depend on details of the species and traits being studied.

Type
Review Article
Information
Genetics Research , Volume 78 , Issue 3 , December 2001 , pp. 213 - 218
Copyright
© 2001 Cambridge University Press