Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-11-24T07:48:45.585Z Has data issue: false hasContentIssue false
  • English
  • Français

A major autosomal gene effect on activity of glucose 6-phosphate dehydrogenase segregating between recombinant inbred lines of mice

Published online by Cambridge University Press:  14 April 2009

Robert P. Erickson  and
Kenneth Harper
Robert P. Erickson
Affiliation:
Department of Human Genetics and Pediatrics, University of Michigan Medical School, 1137 E. Catherine, Ann Arbor, Michigan 48109
Kenneth Harper
Affiliation:
Department of Human Genetics and Pediatrics, University of Michigan Medical School, 1137 E. Catherine, Ann Arbor, Michigan 48109
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

G6PD measurements (expressed per g haemoglobin) in both males and females of the parental lines C57BL/6J and C57L/J and four recombinant inbred (RI) lines between them showed segregation of G6PD levels as parental classes (one line showed the higher C57BL/6J activity, three lines were indistinguishable from C57L/J). Haemoglobin also varied significantly among the parental and RI lines but this variation did not explain the variation in G6PD. NADP levels (expressed per g wet wt of erythrocytes) correlated with G6PD levels but one RI line was intermediate in males while two RI lines were lower than either parental line in females. Thus, the major autosomal locus affecting G6PD levels which segregates between these two inbred lines probably does not act directly on NADP levels. The variation in the G6PD levels in the four RI lines co-segregated with the brown coat color locus but the association is not significant because of the small number of RI lines available.

Type
Research Article
Information
Genetics Research , Volume 36 , Issue 1 , August 1980 , pp. 91 - 97
Copyright
Copyright © Cambridge University Press 1980

References

REFERENCES

Bailey, D. W. (1971). Recombinant-inbred strains: an aid to finding identity, linkage, and function of histocompatibility and other genes. Transplantation 11, 325–327.CrossRefGoogle ScholarPubMed
Blumer, J. L. & Mieyal, J. J. (1978). Genetic expression of microsomal electron transport in mice. Journal of Biological Chemistry 253, 1159–1166.CrossRefGoogle ScholarPubMed
Chapman, V. M. & Shows, T. B. (1976). Somatic cell genetic evidence for X-chromosome linkage of three enzymes in the mouse. Nature 259, 665–667.CrossRefGoogle ScholarPubMed
Ciotti, M. M. & Kaplan, N. D. (1957). Procedures for determination of pyridine nucleotides. Methods in Enzymology. Colowick, S. & Kaplan, N. D., eds., pp. 892–899. New York: Academic Press.Google Scholar
Epstein, C. J., Wegienka, E. O. & Smith, C. W. (1969). Biochemical development of preimplantation mouse embryos: In vivo activities of fructose 1,6-diphosphate aldolase, glucose 6-phosphate dehydrogenase, malate dehydrogenase, and lactate dehydrogenase. Biochemical Genetics 3, 271–281.CrossRefGoogle ScholarPubMed
Erickson, R. P. (1974). Erythrocytic nicotinamide-adenine dinucleotide phosphate levels and the genetic regulation of erythrocytic glucose 6-phosphate dehydrogenase activity in the inbred mouse. Biochemical Genetics 11, 33–40.CrossRefGoogle ScholarPubMed
Erickson, R. P., Stites, D. P., Fudenberg, H. H. & Epstein, C. J. (1972). Altered levels of glucose 6-phosphate dehydrogenase stabilizing factors in X-linked chronic granulomatous disease. Journal of Laboratory Clinical Medicine 80, 644–653.Google ScholarPubMed
Hutton, J. J. (1971). Genetic regulation of glucose 6-phosphate dehydrogenase activity in the inbred mouse. Biochemical Genetics 5, 315–331.CrossRefGoogle ScholarPubMed
Oser, B. L. (ed.) (1955). Hawk's physiological chemistry, 14th ed., p. 1096. New York: McGraw-Hill.Google Scholar
Russell, E. S., Neufeld, E. F. & Higgins, C. T. (1951). Comparison of normal blood picture of young adults from 18 inbred strains of mice. Proceedings of the Society of Experimental Biology and Medicine 78, 761–766.CrossRefGoogle ScholarPubMed
Shire, J. G. M., Kessler, S. & Clayton, R. B. (1972). The availability of NADPH for corticosterone reduction in six strains of mice. Journal of Endocrinology 52, 591–592.CrossRefGoogle ScholarPubMed
Swank, R. T. & Bailey, D. W. (1973). Recombinant inbred lines: value in the genetic analysis of biochemical variants. Science 181, 1249–1252.CrossRefGoogle ScholarPubMed