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Macronuclear DNA in Stentor coeruleus: a first approach to its characterization

Published online by Cambridge University Press:  14 April 2009

B. Pelvat  and
G. De Haller
B. Pelvat
Affiliation:
Laboratoire de Biologie des Protistes, Département de Biologie Animale, Université de Genève, 1211 Genève 4 (Suisse)
G. De Haller
Affiliation:
Laboratoire de Biologie des Protistes, Département de Biologie Animale, Université de Genève, 1211 Genève 4 (Suisse)
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The macronuclei of Stentor coeruleus were isolated on a discontinuous sucrose gradient and their DNA was purified by conventional methods. The GC content was 32 mole%. The DNA banded as a single peak on analytical ultracentrifugation at 1·691 g/cm3. The molecular weight of the DNA was 5 × 106 to 4 × 107 daltons. Genome size determined by DNA–DNA reassociation kinetics was 6 × 1010 daltons. The macronuclear genome was mostly simple, about 85% being made of non-repetitive sequences.

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 2 , April 1976 , pp. 277 - 289
Copyright
Copyright © Cambridge University Press 1976

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