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The lactose system in Klebsiella aerogenes V9A: 3. Specific repression of the lac operon by melibiose and raffinose

Published online by Cambridge University Press:  14 April 2009

E. C. R. Reeve
E. C. R. Reeve
Affiliation:
Institute of Animal Genetics, West Mains Road, Edinburgh EH9 3JN, Scotland
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Summary

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In Klebsiella aerogenes strain V9A melibiose and raffinose fail to induce the lac operon and strongly repress its induction by isopropyl-β-d-thiogalactopyranoside (IPTG). This repression is specific to the lac system and is not released by cAMP, which releases the catabolite repression exerted by glucose and sucrose. It is concluded that melibiose and raffinose bind to the lac repressor molecule in Klebsiella V9A, competitively displacing IPTG, and that the resulting complex is able to repress the lac operon. Other Klebsiella strains tested show the same repression phenomenon.

Type
Short Papers
Information
Genetics Research , Volume 22 , Issue 2 , October 1973 , pp. 217 - 221
Copyright
Copyright © Cambridge University Press 1973

References

REFERENCES

Brown, J. E. & Clarke, P. H. (1970). Mutations in a regulator gene allowing Pseudomonas aeruginosa 8602 to grow on butyramide. Journal of General Microbiology 64, 329–342.Google Scholar
Brenchley, J. E. & Magasanik, B. (1972). Klebsiella aerogenes strain carrying drug-resistance determinants and a lac plasmid. Journal of Bacteriology 112, 200–205.CrossRefGoogle Scholar
Jacob, F. & Monod, J. (1961). Genetic regulatory mechanisms in the synthesis of proteins. Journal of Molecular Biology 3, 318–356.CrossRefGoogle ScholarPubMed
Jobe, A. & Bourgeois, S. (1972). The lac repressor-operator interaction. VII. A repressor with unique binding properties: the X86 repressor. Journal of Molecular Biology 72, 139–152.CrossRefGoogle ScholarPubMed
Leive, L. (1965). Some effects of inducer on synthesis and utilization of β-galactosidase messenger RNA in actinomycin-sensitive Escherichia coli. Biochemical and Biophysical Research Communications 20, 321–327.CrossRefGoogle Scholar
Lester, G. & Bonner, D. M. (1957). Genetic control of raffinose utilisation in Escherichia coli. Journal of Bacteriology 73, 544–552.CrossRefGoogle ScholarPubMed
Müller-Hill, B., Rickenberg, H. V. & Wallenfels, K. (1964). Specificity of induction of the enzymes of the Lac operon in Escherichia coli. Journal of Molecular Biology 10, 303–318.CrossRefGoogle Scholar
Neidhart, F. C. & Magasanik, B. (1957). Reversal of the glucose inhibition of histidase biosynthesis in Aerobacter aerogenes. Journal of Bacteriology 73, 253–259.CrossRefGoogle Scholar
Pardee, A. B. (1957). An inducible mechanism for the accumulation of melibiose in Escherichia coli. Journal of Bacteriology 73, 376–385.CrossRefGoogle ScholarPubMed
Pastan, I. & Perlman, R. L. (1968). The role of the Lac promoter locus in the regulation of β-galactosidase synthesis by cyclic 3′,5′-adenosine monophosphate. Proceedings of the National Academy of Sciences, U.S.A. 61, 1336–1342.CrossRefGoogle Scholar
Prival, M. J. & Magasanik, B. (1971). Resistance to catabolite repression of histidase and proline oxidase during nitrogen-limited growth of Klebsiella aerogenes. Journal of Biological Chemistry 246, 6288–6296.Google ScholarPubMed
Reeve, E. C. R. & Braithwaite, J. A. (1972). The lactose system in Klebsiella aerogenes V9A. 1. Characteristics of two Lac mutant phenotypes which revert to wild-type. Genetical Research 20, 175–191.CrossRefGoogle ScholarPubMed
Reeve, E. C. R. & Braithwaite, J. A. (1973). The lactose system in Klebsiella aerogenes V9A. 2. Galactoside permeases which accumulate lactose or melibiose. Genetical Research 21, 273–285.CrossRefGoogle ScholarPubMed
Schaefler, S. (1967). Isolation of constitutive β-galactosidase permease mutants in Escherichia coli by selection for raffinase fermentation. Bacteriological Proceedings, p. 54.Google Scholar
Schmitt, R. (1968). Analysis of melibiose mutants deficient in α-galactosidase and thiomethyl-galactoside permease II in Escherichia coli K12. Journal of Bacteriology 96, 462–471.CrossRefGoogle Scholar
Williams, B. & Paigen, K. (1968). Paradoxical effect of weak inducers on the lac operon of Escherichia coli. Journal of Bacteriology 96, 447–456.CrossRefGoogle ScholarPubMed