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Investigation of the mating system of Pseudomonas aeruginosa strain 1: IV. Mapping of distal markers

Published online by Cambridge University Press:  14 April 2009

J. S. Loutit
J. S. Loutit
Affiliation:
Department of Microbiology, University of Otago, Dunedin, New Zealand
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Thirteen selected distal markers have been mapped by time of entry studies and linkage analysis. They appear to fall on two distinct linkage groups and with the ten previously described there are nineteen markers on one group and four on the other. There is as yet no clear evidence that the two groups are linked, but this possibility has not been excluded.

Of the thirteen markers described, six can be mapped by measuring their time of entry into suitable recipients. It should now be possible to establish the order of any further marker and to provide more accurate information for any marker transferred within 35 min from the time of mixing the cells.

Type
Research Article
Information
Genetics Research , Volume 13 , Issue 1 , February 1969 , pp. 91 - 98
Copyright
Copyright © Cambridge University Press 1969

References

REFERENCES

Hayes, W., Jacob, F. & Wollman, E. L. (1963). Conjugation in bacteria. Methodology in Basic Genetics, pp. 129–164. Holden-Day Inc.Google Scholar
Loutit, J. S. & Marinus, M. G. (1968). Investigation of the mating system of Pseudomonas aeruginosa strain 1. II. Mapping of a number of early markers. Genet. Res. 12, 37–44.CrossRefGoogle ScholarPubMed
Loutit, J. S., Marinus, M. G. & Pearce, L. E. (1968). Investigation of the mating system of Pseudomonaa aeruginosa strain 1. III. Kinetic studies on the transfer of the sex factor (FP). Genet. Res. 12, 139–145.CrossRefGoogle Scholar
Loutit, J. S., Pearce, L. E. & Marinus, M. G. (1968). Investigation of the mating system of Pseudomonas aeruginosa strain 1. I. Kinetic studies. Genet. Res. 12, 29–36.CrossRefGoogle ScholarPubMed