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Interaction between the plasmid R6K and Escherichia coli with defective DNA polymerase I

Published online by Cambridge University Press:  14 April 2009

D. J. Tweats  and
J. T. Smith
D. J. Tweats
Affiliation:
Genetics Department, University College of Swansea, U.K., and the Microbiology Section, Department of Pharmaceutics, The School of Pharmacy, University of London, U.K.
J. T. Smith
Affiliation:
Genetics Department, University College of Swansea, U.K., and the Microbiology Section, Department of Pharmaceutics, The School of Pharmacy, University of London, U.K.
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Summary

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Initial experiments demonstrated that the plasmid R6K cannot be transferred to or maintained readily in the E. coli DNA polymerase I deficient strain JG138 polA1. Results with E. coli MM386 polA12 (R6K), which has a temperature sensitive polymerase I enzyme, showed cell division becomes abnormal when the polymerase I enzyme of the host bacteria is inactivated at the restrictive temperature. Under conditions of polymerase I deficiency, R6K replication, as measured by monitoring R-factor-mediated β-lactamase activity, also becomes abnormal with the loss of multiple R6K copies per cell and the apparent maintenance of a single R-factor copy per cell.

Type
Research Article
Information
Genetics Research , Volume 32 , Issue 1 , August 1978 , pp. 25 - 35
Copyright
Copyright © Cambridge University Press 1978

References

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