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Insertion-deletion variation at the yellow-achaete-scute region in two natural populations of Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

Robin N. Beech  and
Andrew J. Leigh Brown
Robin N. Beech
Affiliation:
Department of Genetics, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh, EH9 3JN, Scotland
Andrew J. Leigh Brown*
Affiliation:
Department of Genetics, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh, EH9 3JN, Scotland
*
† Corresponding author.
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We have surveyed the region of the X chromosome of Drosophila melanogaster which encodes the yellow, achaete and scute genes for restriction map variation. Two natural populations, one from North Carolina, U.S.A. and the other from southern Spain were screened for variation at about 70 restriction sites and for variation due to DNA insertion or deletion events in 120 kilobases of DNA. Mean heterozygosity per nucleotide was estimated to be 0·0024 and 15 large insertions were found in the 49 chromosomes screened. Extensive disequilibrium between polymorphic sites was found across much of the region in the North Carolina population. The frequency of large insertions, which usually correspond to transposable genetic elements, is significantly lower than has been observed in autosomal regions of the genome. This is predicted for X-linked loci by certain models of transposable element evolution, where copy number is restricted by virtue of the recessive deleterious effects of the insertions. Our results appear to support such models. The deficiency of insertions may in this case be enhanced by hitch-hiking effects arising from the high level of disequilibrium.

Type
Research Article
Information
Genetics Research , Volume 53 , Issue 1 , February 1989 , pp. 7 - 15
Copyright
Copyright © Cambridge University Press 1989

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