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Insertion of the retroposable element, jockey, near the Adh gene of Drosophila melanogaster is associated with altered gene expression

Published online by Cambridge University Press:  14 April 2009

Lisa D. White  and
James W. Jacobson
Lisa D. White*
Affiliation:
Department of Biology, University of Houston, Houston, TX 77204–5513
James W. Jacobson
Affiliation:
Department of Biology, University of Houston, Houston, TX 77204–5513
*
* Corresponding author.
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The alcohol dehydrogenase (Adh) gene of Drosophila melanogaster is well suited to be a gene expression reporter system. Adh produces a measurable phenotype at both the enzyme and mRNA levels. We recovered a spontaneous transposable element (TE) insertion mutation near the Adh gene. The insertion is a truncated retroposable element, jockey, inserted upstream of the adult Adh enhancer region. Comparisons between the Adhjockey allele and its direct wild-type ancestral allele were made in an isogenic background (i.e. identical cis and trans factors). Differences in Adhjockey expression compared with the wild-type can be attributed solely to the presence of the jockey element. This jockey insertion results in a decrease in adult mRNA transcript levels in the Adhjockey homozygous lines relative to the wild-type counterpart and accounts for a correlated decrease in alcohol dehydrogenase (ADH) enzyme activity. The larval ADH activity levels are not detectably different.

Type
Research Article
Information
Genetics Research , Volume 68 , Issue 3 , December 1996 , pp. 203 - 209
Copyright
Copyright © Cambridge University Press 1996

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