Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-08T10:12:32.812Z Has data issue: false hasContentIssue false
  • English
  • Français

Inhibition of respiratory enzyme synthesis in yeast by chloramphenicol: Relationship between chloramphenicol tolerance and resistance to other antibacterial antibiotics

Published online by Cambridge University Press:  14 April 2009

D. Wilkie ,
G. Saunders  and
Anthony W. Linnane
D. Wilkie
Affiliation:
Department of Biochemistry, Monash University, Clayton, Victoria, Australia
G. Saunders
Affiliation:
Department of Biochemistry, Monash University, Clayton, Victoria, Australia
Anthony W. Linnane
Affiliation:
Department of Biochemistry, Monash University, Clayton, Victoria, Australia
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Individual yeast strains show characteristic differences in the amount of chloramphenicol required to inhibit the synthesis of the respiratory system. This varies from 0·05 mg./ml. to 4 mg./ml. A correlation between chloramphenicol and tetracycline resistance appears likely but no correlation between chloramphenicol and erythromycin resistance was observed. These relationships were emphasized by cross-checking spontaneous resistant mutants for each antibiotic. Nearly all mutants showing spontaneous resistance to chloramphenicol had a simultaneous increase in tetracycline resistance but no increase in erythromycin resistance. Spontaneous resistance to erythromycin on the other hand, had no striking effect on tolerance levels to chloramphenicol and tetracycline. Increases in erythromycin resistance were commonly accompanied by an increase in resistance to other macrolide antibiotics. There are similarities between these effects and those described for certain bacterial systems.

Type
Short Papers
Information
Genetics Research , Volume 10 , Issue 2 , October 1967 , pp. 199 - 203
Copyright
Copyright © Cambridge University Press 1967

References

REFERENCES

Brock, T. D. (1961). Chloramphenicol. Boot. Rev. 25, 32–48.Google ScholarPubMed
Clark-Walker, G. D. & Linnane, A. W. (1966). In vivo differentiation of yeast cytoplasmic and mitochondrial protein synthesis with antibiotics. Biochem. biophys. Res. Commun. 25, 8–13.CrossRefGoogle ScholarPubMed
Clark-Walker, G. D. & Linnane, A. W. (1967). Biogenesis of mitochondria in Saccharomyces cerevisiae. J. Cell Biol. 33 (in press).Google Scholar
Huang, M., Biggs, D. R., Clark-Walker, G. D. & Linnane, A. W. (1966). Chloramphenicol inhibition of the formation of participate mitochondrial enzymes of Saccharomyces cerevisiae. Biochim. biophys. Act, 114, 434–436.CrossRefGoogle Scholar
Reeve, E. C. R. (1966). Characteristics of some single-step mutants to chloramphenicol resistance in Escherichia coli K12 and their interactions with R-factor genes. Genet. Res. 7, 281–286.CrossRefGoogle ScholarPubMed
Tanaka, K., Teraska, H., Nagira, T. & Tamaki, M. (1966). 14C erythromycin-ribosome complex and non-enzymatic binding of aminoacyl-transfer RNA to ribosome-messenger RNA complex. Biochim. biophys. Acta, 123, 435.CrossRefGoogle ScholarPubMed
Taubman, S. B., Jones, N. R., Young, F. E. & Corcoran, J. W. (1966). Sensitivity and resistance to erythromycin in Bacillus subtilis 168: the ribosomal binding of erythromycin and chloramphenicol. Biocheim. biophys. Acta, 123, 438–490.Google ScholarPubMed
Vazquez, D. (1966). Binding of chloramphenicol to ribosomes: the effect of a number of antibiotics. Biochim. biophys. Acta, 114, 277–288.CrossRefGoogle ScholarPubMed
Wilkie, D. & Lee, B. K. (1965). Genetic analysis of actidione resistance in Saccharomyces cerevisiae. Genet. Res. 6, 130–138.CrossRefGoogle ScholarPubMed
Wintersberger, E. (1966). In Regulation of Metabolic Processes in Mitochondria. Amsterdam: Elsevier.Google Scholar