Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-28T04:11:02.389Z Has data issue: false hasContentIssue false

The inheritance of the killer character in yeast

Published online by Cambridge University Press:  14 April 2009

J. M. Somers
Affiliation:
Botany Department, Queen Mary College, University of London, London, E. 1
E. A. Bevan
Affiliation:
Botany Department, Queen Mary College, University of London, London, E. 1
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. First- and second-generation crosses between killer, neutral and sensitive strains of yeast have been carried out in all combinations.

2. The results of this analysis indicated that the killer character is under the control of two types of cytoplasmic genetic determinant. One type, (k), determines killing, and the other, (n), neutrality. The absence, (o), of both types of determinants confers the sensitive phenotype.

3. That both types of cytoplasmic determinant require the same dominant nuclear allele, M, for their maintenance has been indicated in two ways. First, both types are lost when the nuclear genotype is changed from M to m. Secondly, cells of genotype m(k) or m(n), which have been shown to occur among the first formed cells arising from spores of Mm(k) and Mm(n) diploids respectively, are unable to maintain their cytoplasmic determinants. On the other hand, spore cultures of M(k) and M(n) genotype derived from these same diploide continue to maintain the determinants.

4. Thus genotype of killer cells is M(k), of neutrals M(n), and of sensitivities either M(o) or m(o).

5. Cells maintaining both types of cytoplasmic determinant (i.e. of genotype M(k)(n) or M−(k)(n)) have been obtained by appropriate crosses, and shown to be of killer phenotype.

6. Alternative hypotheses to account for the results of this genetic analysis have been discussed.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1969

References

REFERENCES

Barer, R. & Saunders-Singer, A. E. (1948). A new single control micromanipulator. Q. Jl microsc. Sci. 89, 437439.Google Scholar
Bevan, E. A. & Costello, W. P. (1964). Preparation of an enzyme which breaks open yeast asci. Microb. Genet. Bull. 21, 5.Google Scholar
Bevan, E. A. & Makower, M. (1963). The physiological basis of the killer character in yeast. Proc. XIth Int. Congr. Genet. 1, 202203. (Abstract.)Google Scholar
Cox, B. S. & Bevan, E. A. (1961). A new technique for isolating spores of yeast. (Saccharomyces cerevisiae). Trans. Br. mycol. Soc. 41, (i) 239242.Google Scholar
Cox, B. S. & Bevan, E. A. (1962). Aneuploidy in yeast. New Phytol. 61, 342355.Google Scholar
Ephrussi, B., Hottinguer, H. & Taulitzki, J. (1949). Action de l'acriflavine sur les levures. II. Etude gentique du mutant ‘petite colonie’. Annls Inst. Pasteur, Paris 76, 417422.Google Scholar
De Fonbrunne, P. (1949). Technique de micromanipulations. Monographie de l'institute Pasteur, Paris.Google Scholar
Lindegren, C. (1949). The Yeast Cell: its Genetics and Cytology. Saint Louis: Educational Publishers, Inc.Google Scholar
Woods, R. A. & Bevan, E. A. (1966). Interallelic complementation at the ad-2 locus of Saccharomyces cerevisiae. Heredity 21, 121130.Google Scholar
Woods, D. R. & Bevan, E. A. (1968). Studies on the nature of the killer factor produced by Saccharomyces cerevisiae. J. gen. Microbiol. 51, 115126.Google Scholar