Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-02T23:45:03.483Z Has data issue: false hasContentIssue false
  • English
  • Français

The induction of gene mutation and chromosome aberration in Chlamydomonas eugametos by a phenylalanine analog*

Published online by Cambridge University Press:  14 April 2009

A. C. McBride  and
C. S. Gowans
A. C. McBride
Affiliation:
The Biological Laboratories, Harvard university, Cambridge, Mass. and Departments of Botany and Genetics, University of Missouri, Columbia, Missouri
C. S. Gowans
Affiliation:
The Biological Laboratories, Harvard university, Cambridge, Mass. and Departments of Botany and Genetics, University of Missouri, Columbia, Missouri

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Evidence is presented which indicates that the phenylalanine analog 2-amino-3-phenylbutanoic acid (APBA) induces mutation to APBA, streptomycin, and neamine resistance in the green alga Chlamydomonas eugametos. Apparent chromosome abnormalities (reciprocal translocations and inversions) were also found among some clones recovered after APBA treatment. The two, mutation and chromosome aberration, are separable.

Type
Research Article
Information
Genetics Research , Volume 14 , Issue 2 , October 1969 , pp. 121 - 126
Copyright
Copyright © Cambridge University Press 1969

References

REFERENCES

Edelson, J. & Keeley, D. F. (1963). Inhibition of thienyl-alanine incorporation in E. coli by 2-amino-3-phenylbutanoic acid. Archs Biochem. Biophys. 103, 175–180.CrossRefGoogle Scholar
Emeis, C. C. (1966). Haploidisierung von diploiden Hefen durch p-Fluoro -phenylalanin. Z. Naturf. 21, 816–817.CrossRefGoogle Scholar
Gillham, N. W. (1965). Induction of chromosomal and nonchromosomal mutations in Chlamydomonas reinhardii with N-methyl-N′-nitro-N-nitrosoguanidine. Genetics 52, 529–537.Google Scholar
Gowans, C. S. (1960). Some genetic investigations on Chlamydomonas eugametos. Z. Vererb-Lehre 91, 63–73.Google ScholarPubMed
Lhoas, P. (1961). Mitotic haploidization by treatment of Aspergillus niger diploide with para-fluorophenylalanine. Nature, Lond. 190, 744.CrossRefGoogle Scholar
Luria, S. E. & Delbrück, M. (1943). Mutations of bacteria from virus sensitivity to virusresistance. Genetics 28, 491–511.Google Scholar
McBride, J. C. & Gowans, C. S. (1967). Pyrithiamine resistance in Chlamydomonas eugametos. Genetics 56, 405–412.Google Scholar
McClintock, B. (1965). The control of gene action in maize. Brookhaven Symp. Biol. 18, 162–184.Google Scholar
Nakamura, K. & Gowans, C. S. (1965). Genetic control of nicotinic acid metabolism in Chlamydomonas eugametos. Genetics 51, 931–945.CrossRefGoogle ScholarPubMed
Perkins, D. D. (1966). Preliminary characterization of chromosome rearrangement using shotasci. Neurospora Newsletter 9, 10–11 (cited by permission).Google Scholar
Sager, R. & Tsubo, Y. (1962). Mutagenic effects of streptomycin in Chlamydomonas. Arch. Mikrobiol. 42, 159–175.Google Scholar
Starr, R. C. (1964). The culture collection of algae at Indiana University. Am. J. Bot. 51, 67–86.CrossRefGoogle Scholar
Strömnaes, O. (1968). Genetic changes in Saccharomyces cerevisiae grown on media containing dl-pora-fluoro-phenylalanine. Hereditas 59, 197–220.Google Scholar