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Immunogenetic analysis of proteins of Paramecium V. Detection of specific determinants in strains lacking a surface antigen*

Published online by Cambridge University Press:  14 April 2009

Irving Finger  and
Carol Heller
Irving Finger
Affiliation:
Biology Department, Haverford College, Haverford, Pennsylvania, U.S.A.
Carol Heller
Affiliation:
Biology Department, Haverford College, Haverford, Pennsylvania, U.S.A.
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Several stocks of Paramecium aurelia that had never expressed an E serotype were examined for E-reacting material. The regular presence of the cross-reacting antigen G in these stocks ruled out the use of hapten inhibition and other standard immunological techniques. Consequently, a genetic approach was attempted in which hybrids of the E-deficient strains and non-E stocks were bred, and antigen isolated and tested for the existence of molecules with a specificity differing from that of the E-containing parent.

Gel diffusion analysis suggested that E antigen from a hybrid indeed could differ from the ‘normal’ E. One of these hybrid clones which behaved as though its E antigen may have possessed a determinant contributed by the E-deficient strain was used as an immunizing antigen. The antibodies elicited possessed specificity unique for the hybrid. Thus the possession of E-like determinants by E-deficient stocks was confirmed.

Type
Research Article
Information
Genetics Research , Volume 5 , Issue 1 , February 1964 , pp. 127 - 136
Copyright
Copyright © Cambridge University Press 1964

References

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