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Identification of two genes controlling kasugamycin resistance in the filamentous fungus Podospora anserina

Published online by Cambridge University Press:  14 April 2009

An Kieu-Ngoc  and
Evelyne Coppin-Raynal
An Kieu-Ngoc
Affiliation:
Laboraloires de Génétique (UA 86 au CNRS)Université de Paris-Sud, Bâtiment 400 91405 ORSAY Cedex – France
Evelyne Coppin-Raynal*
Affiliation:
Laboraloires de Génétique (UA 86 au CNRS)Université de Paris-Sud, Bâtiment 400 91405 ORSAY Cedex – France
*
† Corresponding author.
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Summary

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We have investigated the effect of the ribosome-targeted antibiotic kasugamycin (ksg) in Podospora anserina. While ksg inhibits both growth and sporulation, it has a stronger inhibitory effect on the sporulation process. It was previously reported that sporulation of Podospora could be impaired when ribosomes translate with a too high accuracy, and since ksg was demonstrated to increase the ribosomal accuracy in E. coli, we wondered whether it would act similarly in Podospora. As a first approach we have isolated two mutations at different loci, Ks1 and Ks2, that increase the resistance to ksg at the level of both growth and sporulation. Interestingly Ks1−1 also confers a decreased resistance to paromomycin, which is a mistranslation inducer. Characterization of Ks1−1 and Ks2−1 mutants suggests that they could be ribosomal mutants.

Type
Research Article
Information
Genetics Research , Volume 51 , Issue 3 , June 1988 , pp. 179 - 184
Copyright
Copyright © Cambridge University Press 1988

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