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Heterochromatin as a factor affecting X-inactivation in interspecific female vole hybrids (Microtidae, Rodentia)

Published online by Cambridge University Press:  14 April 2009

Suren M. Zakian ,
Tatyana B. Nesterova ,
Olga V. Cheryaukene  and
Michail N. Bochkarev
Suren M. Zakian*
Affiliation:
Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk 630090, USSR
Tatyana B. Nesterova
Affiliation:
Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk 630090, USSR
Olga V. Cheryaukene
Affiliation:
Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk 630090, USSR
Michail N. Bochkarev
Affiliation:
Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk 630090, USSR
*
* Corresponding author.
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Summary

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Female interspecific vole hybrids were examined for the expression of the G6PD and GALA genes on the X chromosomes. When one of the parents was a species with a heterochromatin block on the X, and the other parent was M. arvalis, without a heterochromatin block on the X, preferential expression of the genes of the M. arvalis X was consistently observed. When both parental species had heterochromatin on the X, the parental forms of G6PD and GALA were in about equal proportions in the hybrid females. The results of the cytological identification of the active and inactive X on the metaphase spreads in the hybrid females are in agreement with the biochemical results. It is suggested that the observed phenomenon may be due to a nonrandom inactivation of the X chromosome containing a heterochromatin block in crosses involving M. arvalis and by a random inactivation in those with both parents having heterochromatin blocks on the X chromosomes. These results support our previous suggestion that heterochromatin has an effect on X inactivation in female interspecific vole hybrids.

Type
Research Article
Information
Genetics Research , Volume 58 , Issue 2 , October 1991 , pp. 105 - 110
Copyright
Copyright © Cambridge University Press 1991

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