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Genetics of Aspergillus flavus: complementation and mapping of aflatoxin mutants

Published online by Cambridge University Press:  14 April 2009

K. E. Papa
K. E. Papa
Affiliation:
Department of Plant Pathology and Plant Genetics, University of Georgia, Athens, Georgia 30602, U.S.A.
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Summary

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Mutants of Aspergillus flavus impaired in aflatoxin production were induced with N-methyl-N′-nitrosoguanidine and analysed by means of the parasexual cycle. The gene symbol afl was assigned to this type of mutation. Diploid complementation tests revealed that most of 14 afl mutants belonged to different complementation groups. One mutant (afl-1) failed to complement or only partially complemented all other mutants. Haploidization of one diploid revealed the independent segregation of two afl mutants. Heterozygous diploids were synthesized between two afl mutants and tester strains genetically labelled on eight linkage groups. Haploidization of the diploids led to the assignment of two aft mutants to linkage groups. Linkage of afl-4 to w+ and afl-1 to leu on linkage groups II and VII, respectively, was demonstrated.

Type
Research Article
Information
Genetics Research , Volume 34 , Issue 1 , August 1979 , pp. 1 - 9
Copyright
Copyright © Cambridge University Press 1979

References

REFERENCES

Donkersloot, J. A., Mateles, R. I. & Yang, S. S. (1972). Isolation of averufin from a mutant of Aspergillus parasiticus impaired in aflatoxin biosynthesis. Biochemical and Biophysical Research Communications 47, 1051–1055.CrossRefGoogle ScholarPubMed
Gussack, G., Bennett, J. W., Cavalier, S. & Yatsu, L. (1977). Evidence for the parasexual cycle in a strain of Aspergillus flavus containing virus-like particles. Mycopathologia 61, 159–166.CrossRefGoogle Scholar
Hara, S., Fennell, D. I. & Hesseltine, C. W. (1974). Aflatoxin-producing strains of Aspergillus flavus detected by fluorescence of agar medium under ultraviolet light. Applied Microbiology 27, 1118–1123.CrossRefGoogle ScholarPubMed
Heathcote, J. G., Dutton, M. F. & Hibbert, J. R. (1976). Biosynthesis of aflatoxins, Part II. Chemistry and Industry 20, 270–272.Google Scholar
Holt, G., Edwards, G. F. St L. & Macdonald, K. D. (1976). The genetics of mutants impaired in the biosynthesis of penicillin. Proceedings of the Second International Symposium on the Genetics of Industrial Microorganisms (ed. Macdonald, K. D.), pp. 199–211. New York and London: Academic Press.Google Scholar
Kohli, J., Hottinger, H., Munz, P., Strauss, A. & Thuriaux, P. (1977). Genetic mapping in Schizosaccharomyces pombe by mitotic and meiotic analysis and induced haploidization. Genetics 87, 471–489.CrossRefGoogle ScholarPubMed
Leaich, L. L. & Papa, K. E. (1974). Aflatoxins in mutants of Aspergillus flavus. Mycopathologia et Mycologia Applicata 52, 223–229.CrossRefGoogle ScholarPubMed
Lee, L. S., Bennett, J. W., Cucullu, A. F. & Stanley, J. B. (1975). Synthesis of vericolorin A by a mutant strain of Aspergillus parasiticus deficient in aflatoxin production. Agricultural and Food Chemistry 23, 1132–1134.CrossRefGoogle Scholar
Lee, L. S., Bennett, J. W., Goldblatt, L. A. & Lundin, R. E. (1971). Norsolorinic acid from a mutant strain of Aspergillus parasiticus. Journal of the American Oil Chemists Society 48, 93–94.CrossRefGoogle ScholarPubMed
Lillard, H. S., Hanlin, R. T. & Lillard, D. A. (1970). Aflatoxigenic isolates of Aspergillus flavus from pecans. Applied Microbiology 19, 128–130.CrossRefGoogle ScholarPubMed
Papa, K. E. (1973). The parasexual cycle in Aspergillus flavus. Mycologia 65, 1201–1205.CrossRefGoogle ScholarPubMed
Papa, K. E. (1976). Linkage groups in Aspergillus flavus. Mycologia 68, 159–165.CrossRefGoogle ScholarPubMed
Papa, K. E. (1977). Genetics of aflatoxin production in Aspergillus flavus: linkage between a gene for a high B2:B1 ratio and the histidine locus on linkage group VIII. Mycologia 69, 1185–1190.Google ScholarPubMed
Papa, K. E. (1978). The parasexual cycle in Aspergillus parasiticus. Mycologia 70, 766–773.CrossRefGoogle ScholarPubMed
Singh, R. & Hsieh, D. P. H. (1977). Aflatoxin biosynthetic pathway: elucidation by using blocked mutants of Aspergillus parasiticus. Archives of Biochemistry and Biophysics 178, 285–292.CrossRefGoogle ScholarPubMed
Snedecor, G. W. (1956). Statistical Methods, pp. 1–534. Ames: Iowa State College Press.Google Scholar