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Genetics and biochemistry of 5-Bromodeoxyuridine resistance in Physarum polycephalum

Published online by Cambridge University Press:  14 April 2009

Asgeir Lunn ,
David Cooke  and
Finn Haugli
Asgeir Lunn
Affiliation:
Institute of Medical Biology, University of Tromsø, 9000 Tromsø, Norway
David Cooke
Affiliation:
Institute of Medical Biology, University of Tromsø, 9000 Tromsø, Norway
Finn Haugli
Affiliation:
Institute of Medical Biology, University of Tromsø, 9000 Tromsø, Norway

Summary

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5-Bromodeoxyuridine (BrdU)-resistant mutants of Physarum polycephalum were isolated as colonies of myxamoebae growing on BrdU-substituted bacteria after exposure to long-wave ultraviolet light (UV). Twenty-four such mutants were studied. They all show Mendelian segregation in crosses with wild type. Plasmodia constructed from mutant amoebae were all deficient in deoxythymidine incorporation. Extracts made from selected plasmodia showed that all except one had low thymidine kinase activity.

Genetical and biochemical complementation studies revealed two complementation groups: 23 mutants, bur A, had low thymidine kinase while 1 mutant, bur B, had normal thymidine kinase levels.

Type
Research Article
Information
Genetics Research , Volume 30 , Issue 1 , August 1977 , pp. 1 - 12
Copyright
Copyright © Cambridge University Press 1977

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