Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-12T21:27:03.986Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetical and structural analyses of cell-wall formation in Chlamydomonas reinhardi

Published online by Cambridge University Press:  14 April 2009

D. Roy Davies  and
A. Plaskitt
D. Roy Davies
Affiliation:
John Innes Institute, Norwich NOR 70F
A. Plaskitt
Affiliation:
John Innes Institute, Norwich NOR 70F
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Fifteen mutant strains of Chlamydomonas reinhardi were isolated which showed defects in some aspect of the process of cell-wall formation. Genetic analyses indicated that most of the mutations were due to single gene changes; two were anomalous in that non-Mendelian segregations were obtained on crossing with other genotypes, and on selfing they frequently gave rise to wild-type phenotypes.

Occasional somatic revertants were also obtained from these two strains. On the basis of these analyses it is suggested that there are two levels of control operating in the process of cell wall biogenesis - one concerned with subunit production at the nuclear level and another, possibly concerned with three-dimensional organization, at another level. Electron-microscope analyses of the different mutants showed the mutants to be divided into three main categories: those in which the wall was formed but was not attached to the plasma membrane, those in which the wall was attached to the membrane, and those in which very little wall was formed. In the last class in particular, vesicles containing wall precursors were clearly visible, and were shed through the plasma membrane into the medium.

Type
Research Article
Information
Genetics Research , Volume 17 , Issue 1 , February 1971 , pp. 33 - 43
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Beisson, J. & Sonneborn, T. M. (1965). Cytoplasmic inheritance of the organization of the cell cortex in Paramecium aurelia. Proceedings of the National Academy of Sciences, U.S.A. 53, 275–282.Google Scholar
Cox, B. S. (1965). A cytoplasmic suppressor of supersuppressor in yeast. Heredity 20, 505–521.Google Scholar
Ebersold, W. T. (1967). Chlamydomonas reinhardi; heterozygous diploid strains. Science 157, 447–449.Google Scholar
Horne, R. W. & Davis, D. R. (1970). High resolution electron microscopy and optical diffraction studies of Chlamydomonas cell walls. Septième Congrès International de Microscopie Electronique, Grenoble, p 117.Google Scholar
Jinks, J. L. (1963). Cytoplasmic inheritance in fungi. In Methodology in Basic Genetics (ed. Burdette, W. J.), pp. 325–354. Holden-Day, Inc.Google Scholar
Kates, J. R. & Jones, R. F. (1964). Control of gametic differentiation in liquid cultures of Chlamydomonas. Journal of Cellular and Comparative Physiology 63, 157–164.CrossRefGoogle Scholar
Lawrence, C. W. & Davies, D. R. (1967). The mechanism of recombination in Chlamydomonas reinhardi: the influence of inhibitors of protein synthesis on intergenic recombination. Mutation Research. 4, 137–146.Google Scholar
Levine, M. (1969). Phage morphogenesis. In Annual Review of Genetics, vol. 3 (editor Roman, H. L.), pp. 232–342.Google Scholar
Lhoas, P. (1961). Mitotic haploidization by treatment of Aspergittus niger diploids with para-fluorophenylalanine. Nature, London 190, 744.Google Scholar
Luft, J. H. (1961). Improvements in epoxy resin embedding methods. Journal of Biophysical and Biochemical Cytology 9, 409–414.Google Scholar
Luftig, R. (1967). Catalase crystals as an internal marker. Journal of Ultrastructural Research 20, 91–102.CrossRefGoogle ScholarPubMed
Millonig, G. (1961). Modified procedure for lead staining of thin sections. Journal of Biophysical and Biochemical Cytology 11, 736–739.Google Scholar
Muhlethaler, K. (1967). Ultrastructure and formation of plant cell walls. In Annual Review of Plant Physiology 18, 1–24.Google Scholar
Randall, J. (1969). The flagellar apparatus as a model organelle for the study of growth and morphopoiesis. Proceedings of the Royal Society B 173, 31–62.Google Scholar
Sager, R. & Granick, S. (1954). Nutritional control of sexuality in Chlamydomonas reinhardi. Journal of General Physiology 37, 729–742.Google Scholar
Sager, R. & Palade, G. E. (1957). Structure and development of the chloroplast in Chlamydomonas. Journal of Biophysical and Biochemical Cytology 3, 463–488.CrossRefGoogle ScholarPubMed
Sager, R. & Ramanis, Z. (1970). A genetic map of non-Mendelian genes in Chlamydomonas. Proceedings of the National Academy of Sciences U.S.A. 65, 593–600.Google Scholar
Sueoka, N. (1960). Mitotic replication of deoxyribonucleic acid in Chlamydomonas reinhardi. Proceedings of the National Academy of Sciences U.S.A. 46, 83–91.CrossRefGoogle ScholarPubMed