Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-25T16:21:54.266Z Has data issue: false hasContentIssue false
  • English
  • Français

The genetic locations of traO, finP and tra-4 on the E. coli K12 sex factor F

Published online by Cambridge University Press:  14 April 2009

Neil Willetts ,
John Maule  and
Sarah McIntire
Neil Willetts
Affiliation:
Department of Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR
John Maule
Affiliation:
Department of Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR
Sarah McIntire
Affiliation:
Department of Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Using a series of Hfr and F prime deletion strains, the F transfer inhibition gene finP has been mapped between ori and traJ. Marker rescue experiments with the Hfr deletion strains further showed that traO, the site of action of the transfer inhibitor, is located immediately to the left of, or possibly within, traJ, and that the polar mutation tra-4 lies in traK.

Type
Research Article
Information
Genetics Research , Volume 26 , Issue 3 , December 1975 , pp. 255 - 263
Copyright
Copyright © Cambridge University Press 1975

References

REFERENCES

Achtman, M., Willetts, N. S. & Clark, A. J. (1971). Beginning a genetic analysis of conjugational transfer determined by the F factor in E. coli by isolation and characterisation of transfer-deficient mutants. Journal of Bacteriology 106, 529–538.Google Scholar
Achtman, M., Willetts, N. S. & Clark, A. J. (1972). A conjugational complementation analysis of transfer-deficient mutants of Flac in E. coli. Journal of Bacteriology 110, 831–842.Google Scholar
Finnegan, D. J. & Willetts, N. S. (1971). Two classes of Flac mutants insensitive to transfer inhibition by an F-like R factor. Molecular and general Genetics 111, 256–264.Google Scholar
Finnegan, D. J. & Willetts, N. S. (1972). The nature of the transfer inhibitor of several F-like plasmids. Molecular and general Genetics 119, 57–66.Google Scholar
Finnegan, D. J. & Willetts, N. S. (1973). The site of action of the F transfer inhibitor. Molecular and general Genetics 127, 307–316.Google Scholar
Gasson, M. J. & Willetts, N. S. (1975). Five control systems preventing transfer of E. coli K12 sex factor F. Journal of Bacteriology 122, 518–525.Google Scholar
Guyer, M. & Clark, A. J. (1975). Isolation and characterisation of cis-dominant transfer-deficient mutants of the F sex factor of E. coli K12. Proceedings of the National Academy of Sciences, U.S. (in the Press).Google Scholar
Helmuth, R. & Achtman, M. (1976). Operon structure of DNA transfer cistrons on the F sex factor. Nature 257, 652–656.CrossRefGoogle Scholar
Ippen-Ihler, K., Achtman, M. & Willetts, N. S. (1972). A deletion map of the E. coli K12 sex factor F: the order of eleven transfer cistrons. Journal of Bacteriology 110, 857–863.Google Scholar
Willetts, N. S. & Achtman, M. (1972). A genetic analysis of transfer by the E. coli sex factor F, using PI transductional complementation. Journal of Bacteriology 110, 843–851.CrossRefGoogle Scholar
Willetts, N. S. (1972). Location of the origin of transfer of the sex factor F. Journal of Bacteriology 112, 773–778.CrossRefGoogle ScholarPubMed
Willetts, N. S. (1974 a). Mapping loci for surface exclusion and incompatibility on the F factor of E. coli K12. Journal of Bacteriology 118, 778–782.Google Scholar
Willetts, N. S. (1974 b). The kinetics of inhibition of Flac transfer by R100 in E. coli. Molecular and general Genetics 129, 123–130.CrossRefGoogle ScholarPubMed