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The genetic locations of traO, finP and tra-4 on the E. coli K12 sex factor F

Published online by Cambridge University Press:  14 April 2009

Neil Willetts
Affiliation:
Department of Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR
John Maule
Affiliation:
Department of Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR
Sarah McIntire
Affiliation:
Department of Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR
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Summary

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Using a series of Hfr and F prime deletion strains, the F transfer inhibition gene finP has been mapped between ori and traJ. Marker rescue experiments with the Hfr deletion strains further showed that traO, the site of action of the transfer inhibitor, is located immediately to the left of, or possibly within, traJ, and that the polar mutation tra-4 lies in traK.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1975

References

REFERENCES

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