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Genetic investigation of a negatively phototactic strain of Chlamydomonas reinhardtii

Published online by Cambridge University Press:  14 April 2009

Robert D. Smyth  and
W. T. Ebersold
Robert D. Smyth
Affiliation:
Department of Physics, Syracuse University, Syracuse, New York 13210
W. T. Ebersold
Affiliation:
Department of Biology, University of California, Los Angeles, California 90024
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Two laboratory strains of the green alga Chlamydomonas reinhardtii137c differ in their pattern of phototactic aggregation. One is positively phototactic under conditions where the other is negatively phototactic. The trait segregates 2:2 in tetrads and maps to a single locus. Heterozygous diploids are positively phototactic, showing that this allele is dominant. The aggregation pattern caused by either allele is not altered by the introduction of an unlinked gene that suppresses development of the eyespot. Probably the strains already differed in phototactic behaviour at the time they were first isolated. They may therefore reflect a genetic polymorphism common among soil algae. The genetic data allowed another significant observation not specifically related to phototaxis. Anomalous products from some crosses suggest that four nuclei sometimes fuse into a tetraploid zygote that then undergoes meiosis. The meiotic products that result are diploid. This represents a previously undescribed mechanism of diploid formation in Chlamydomonas.

Type
Research Article
Information
Genetics Research , Volume 46 , Issue 2 , October 1985 , pp. 133 - 148
Copyright
Copyright © Cambridge University Press 1985

References

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