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Genetic and molecular analyses of vgal: a spontaneous and unstable mutation at the vestigial locus in Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

Claude Bazin ,
Françoise Lemeunier ,
Georges Periquet  and
Joël Silber
Claude Bazin*
Affiliation:
Laboratoire de Génétique Quantitative et Moléculaire, Université Paris 7, CNRS URA 693, France
Françoise Lemeunier
Affiliation:
Laboratoire de Biologie et Génétique Evolutives, CNRS UPR 2411–Gif sur Yvette, France
Georges Periquet
Affiliation:
IBEA, Université F Rabelais, CNRS URA 1298–372000 Tours, France
Joël Silber
Affiliation:
Laboratoire de Génétique Quantitative et Moléculaire, Université Paris 7, CNRS URA 693, France
*
* Corresponding author.
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Summary

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We describe herein, a new unstable mutant of the vestigial locus, isolated from a French natural population. From this mutant vestigialalmost (vgal) wild-type flies (vgal+) and extreme vg phenotypes (vge) arose spontaneously without genomic shock. The occurrence of vgal+ or vge alleles depends mostly on the breeding temperature; vgal+ revertants arose principally at low temperature (21 °C) and vge at 28 °C. These events occur mainly in the male germ line and the phenomenon appears to be premeiotic. Our results with in situ hybridization experiments and Southern blots show that the vgal mutation is due to a 2 kb DNA insertion, which is a deleted hobo element. Genetic and molecular analyses show that two distinct events may underly the wild-type revertants. One is the excision of the resident hobo element, the other a further deletion (about 300 bp in the example characterized herein). The vge mutation is probably due to a deletion of vestigial sequences flanking the hobo insertion.

Type
Research Article
Information
Genetics Research , Volume 57 , Issue 3 , June 1991 , pp. 235 - 243
Copyright
Copyright © Cambridge University Press 1991

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