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Genetic analysis of the phosphatases in Aspergillus nidulans

Published online by Cambridge University Press:  14 April 2009

G. Dorn
G. Dorn
Affiliation:
Department of Genetics, The University, Glasgow*
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1. A histochemical method has been applied to the detection of alkaline and acid phosphatase mutants in single colonies of Aspergillus nidulans.

2. With the above method it has been possible to isolate mutants in which the alkaline and acid phosphatase activities are affected either separately or simultaneously.

3. Crude extracts of wild-type A. nidulans contain four electrophoretically distinct phosphatase components, two with activity at alkaline pH and two with activity at acid pH. Genes affecting three of the four components have been identified.

4. Two suppressor mutants of an alkaline phosphataseless mutant (palB7) have been isolated. In a strain carrying palB7 and one of these suppressors, the restoration of an alkaline phosphatase component is accompanied by loss of the faster acid phosphatase component. In a similar strain carrying the other suppressor, the partial restoration of the alkaline phosphatase component goes with an electrophoretic alteration of the slower acid phosphatase component.

5. Genetic analysis of twenty-seven mutants has resulted in the identification of fifteen loci affecting the phosphatases. All these loci have been assigned to linkage groups, and twelve of them were also mapped meiotically in relation to other loci.

6. One possible model (based on heteropolymeric proteins) has been proposed to account for the electrophoretic and genetic data on the various phosphatase and suppressor mutations.

Type
Research Article
Information
Genetics Research , Volume 6 , Issue 1 , February 1965 , pp. 13 - 26
Copyright
Copyright © Cambridge University Press 1965

References

REFERENCES

Apirion, D. (1963). Studies on the selection of mutants in Aspergillus nidulans. Ph.D. Thesis, Glasgow University.Google Scholar
Echols, H., Garen, A., Garen, S. & Torriani, A. (1961). Genetic control of repression of alkaline phosphatase in E. coli. J. Molec. Biol. 3, 425–438.CrossRefGoogle ScholarPubMed
Forbes, E. (1959). Use of mitotic segregation for assigning genes to linkage groups in Aspergillus nidulans. Heredity, 13, 67–80.CrossRefGoogle Scholar
Forbes, E. (1963). A strain with all chromosomes marked for use in haploidization. Aspergillus News Letter, 4, 15.Google Scholar
Garen, A. (1960). Genetic control of the specificity of the bacterial enzyme alkaline phos-phatase. Microbial Genetics, 10th Symp. Soc. Gen. Microbiol. Cambridge: The University Press. pp. 239–247.Google Scholar
Garen, A. & Levinthal, C. (1960). A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. Biochim. biophys. Acta, 38, 470–483.CrossRefGoogle ScholarPubMed
Jacob, F. & Monod, J. (1961). Genetic regulatory mechanisms in the synthesis of proteins. J. Molec. Biol. 3, 318–356.CrossRefGoogle ScholarPubMed
Käfer, E. (1958). An 8-chromosome map of Aspergillus nidulans. Advanc. Genet. 9, 105–145.CrossRefGoogle ScholarPubMed
Lhoas, P. (1961). Mitotic haploidization by treatment of Aspergillus niger diploids with para-fluoro-phenylalanine. Nature, 190, 744.CrossRefGoogle Scholar
Morpurgo, G. (1961). Somatic segregation by p-fluoro-phenylalanine. Aspergillus News Letter, 2, 10.Google Scholar
Pearse, A. G. Everson (1960). Alkaline and acid phosphatases. Histochemistry, pp. 384–455. London: J. & A. Churchill Ltd.Google Scholar
Pontecorvo, G. (1956). The parasexual cycle in fungi. Annu. Rev. Microbiol. 10, 393–400.CrossRefGoogle ScholarPubMed
Pontecorvo, G. & Käfer, E. (1958). Genetic analysis based on mitotic recombination. Advanc. Genet. 9, 71–104.CrossRefGoogle ScholarPubMed
Pontecorvo, G., Roper, J. A., Hemmons, L. M., Macdonald, K. D. & Bufton, A. W. J. (1953). The genetics of Aspergillus nidulans. Advanc. Genet. 5, 141–238.CrossRefGoogle ScholarPubMed
Pontecorvo, G., Tarr Gloor, E. & Forbes, E. (1954). Analysis of mitotic recombination in Aspergillus nidulans. J. Genet. 52, 226–237.CrossRefGoogle Scholar
Poulik, M. D. (1957). Starch gel electrophoresis in a discontinuous system of buffers. Nature, 180, 1477–1479.CrossRefGoogle Scholar
Roberts, C. F. (1963). The genetic analysis of carbohydrate utilization in Aspergillus nidulans. J. gen. Microbiol. 31, 45–58.CrossRefGoogle ScholarPubMed
Roche, Jean (1950). Phosphatases. The Enzymes, vol. 1, pp. 473–516. New York: Academic Press Inc.Google Scholar
Smithies, O. (1955). Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults. Biochem. J. 61, 629–641.CrossRefGoogle ScholarPubMed
Torriani, A. (1960). Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli. Biochim. biophys. Acta, 38, 460–469.CrossRefGoogle ScholarPubMed