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Genetic analysis of morphogenetic processes in Paramecium: I. A mutation affecting trichocyst formation and nuclear division

Published online by Cambridge University Press:  14 April 2009

Françoise Ruiz ,
André Adoutte ,
Michèle Rossignol  and
Janine Beisson
Françoise Ruiz
Affiliation:
Centre de Génétique Moléculaire, CNRS, 91190 Gif-sur-Yvette, France
André Adoutte
Affiliation:
Centre de Génétique Moléculaire, CNRS, 91190 Gif-sur-Yvette, France
Michèle Rossignol
Affiliation:
Centre de Génétique Moléculaire, CNRS, 91190 Gif-sur-Yvette, France
Janine Beisson
Affiliation:
Centre de Génétique Moléculaire, CNRS, 91190 Gif-sur-Yvette, France
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Summary

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Mutation tam38 of Paramecium tetraurelia is a nuclear recessive mutation with a pleiotropic effect on both trichocyst morphogenesis and nuclear processes. The analysis of the defective nuclear processes (micronuclear and macronuclear divisions, nuclear reorganization at autogamy) shows that these defects result from an abnormal localization of the nuclei. Phenocopies of tam38 abnormalities can be obtained by vinblastine treatment of wild-type cells at late stages of division. Taking into account the similarity between tam38 and a series of other mutations which also prevent trichocyst attachment to the cell surface and disturb nuclear divisions, the following interpretation is proposed: the absence of attached trichocyst induces structural changes in the plasma membrane or in the cortical region which disturb the normal cortical control of the localization of nuclei.

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 2 , April 1976 , pp. 109 - 122
Copyright
Copyright © Cambridge University Press 1976

References

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