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Gene transfer in Drosophila melanogaster: genetic transformations induced by the DNA of transformed stocks

Published online by Cambridge University Press:  14 April 2009

Allen S. Fox ,
Sheldon D. Parzen ,
Helen Salverson  and
Sei Byung Yoon
Allen S. Fox
Affiliation:
Laboratory of Genetics, University of Wisconsin, Madison, Wisconsin 53706, U.S.A.
Sheldon D. Parzen
Affiliation:
Laboratory of Genetics, University of Wisconsin, Madison, Wisconsin 53706, U.S.A.
Helen Salverson
Affiliation:
Laboratory of Genetics, University of Wisconsin, Madison, Wisconsin 53706, U.S.A.
Sei Byung Yoon
Affiliation:
Laboratory of Genetics, University of Wisconsin, Madison, Wisconsin 53706, U.S.A.
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DNA prepared from transformed stocks of Drosophila melanogaster induces second-step transformations resembling the original. The gross yield of transformants induced by transformed DNA is several times higher than that induced by the original allo-DNA, but much of this high frequency is attributable to a few exceptionally large clusters of transformants among flies treated with transformed DNA. When these large clusters are omitted from the data, the frequency of transformants induced by DNA from transformed stocks is the same as that induced by allo-DNA. The data therefore support the conclusion that the original DNA-induced alterations resulted from the transfer of genetic material capable of indefinite replication.

Type
Research Article
Information
Genetics Research , Volume 26 , Issue 2 , October 1975 , pp. 137 - 147
Copyright
Copyright © Cambridge University Press 1975

References

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