Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-24T06:57:41.296Z Has data issue: false hasContentIssue false
  • English
  • Français

The frequency in Neurospora tetrads of multiple exchanges within short intervals*†

Published online by Cambridge University Press:  14 April 2009

David D. Perkins
David D. Perkins
Affiliation:
Department of Biological Sciences, Stanford University, Stanford, California, U.S.A.
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Tetrad data from short gene-marked intervals provide information on the frequency of multiple exchanges within intervals. Non-parental ditype and tetratype frequencies from 58,000 interval-tetrads of Neurospora crassa show that 4-strand double exchanges are far less frequent than would be expected in the absence of chiasma or chromatid interference. These results are in general agreement with meiotic tetrad data from other organisms, except Aspergillus nidulans. They preclude the occurrence of reciprocal meiotic exchanges as clusters unless multiples within each cluster are restricted so as not to involve all four chromatids. If this is not the case, and chromatid interference does not occur, then chiasma interference must be strongly positive within short regions. Known cases of apparent negative interference among random meiotic segregants are probably the result of non-reciprocal conversion of a middle marker, rather than of multiple reciprocal crossing over.

Type
Research Article
Information
Genetics Research , Volume 3 , Issue 2 , July 1962 , pp. 315 - 327
Copyright
Copyright © Cambridge University Press 1962

References

REFERENCES

Barratt, R. W. & Ogata, W. (1954). A strain of Neurospora with an alternative requirement for leucine or aromatic amino acids. Amer. J. Bot. 41, 763–771.CrossRefGoogle Scholar
Barratt, R. W., Newmeyer, D., Perkins, D. D. & Garnjobst, L. (1954). Map construction in Neurospora crassa. Advanc. Genet. 6, 1–93.CrossRefGoogle ScholarPubMed
Boone, D. M. & Keitt, G. W. (1956). Venturia inaequalis(Cke.)Wint. VIII. Inheritance of color mutant characters. Amer. J. Bot. 43, 226–233.CrossRefGoogle Scholar
Bole-Gowda, B. N., Perkins, D. D. & Strickland, W. N. (1962). Crossing over and interference in the centromere region of linkage group I of Neurospora. (In. preparation.)CrossRefGoogle Scholar
Case, M. E. & Giles, N. H. (1958). Recombination mechanisms at the pan-2 locus in Neurospora crassa. Cold Spr. Harb. Symp. quant. Biol. 23, 119–135.CrossRefGoogle ScholarPubMed
Day, P. R. & Anderson, G. E. (1961). Two linkage groups in Coprinus lagopus. Genet. Res. 2, 414–423.CrossRefGoogle Scholar
Desborough, S. & Lindegren, G. (1959). Chromosome mapping of linkage data from Saccharomyces by tetrad analysis. Genetica, 30, 346–383.CrossRefGoogle ScholarPubMed
Desborough, S., Shult, E. E., Yoshida, T. & Lindegren, C. C. (1960). Interference patterns in family Y-1 of Saccharomyces. Genetics, 45, 1467–1480.CrossRefGoogle ScholarPubMed
Dubes, G. R. (1953). Investigations of some ‘unknown’ mutants of Neurospora crassa. Ph.D. Thesis, Calif. Inst. Technol.Google Scholar
Ebersold, W. T. (1956). Crossing over in Chlamydomonas reinhardi. Amer. J. Bot. 43, 408–410.CrossRefGoogle Scholar
Ebersold, W. T. & Levine, R. P. (1959). A genetic analysis of linkage group I of Chlamydomonas reinhardi. Z. indukt. Abstamm.- u. Vererb Lehre, 90, 74–82.Google Scholar
El-Ani, A. S., Olive, L. S. & Kitani, Y. (1961). Genetics of Sordaria fimicola. IV. Linkage group I. Amer. J. Bot. 48, 716–723.CrossRefGoogle Scholar
Eversole, R. A. (1956). Biochemical mutants of Chlamydomonas reinhardi. Amer. J. Bot. 43, 404–407.CrossRefGoogle Scholar
Eversole, R. A. & Tatum, E. L. (1956). Chemical alteration of crossing-over frequency in Chlamydomonas. Proc. nat. Acad. Sci., Wash., 42, 68–73.CrossRefGoogle ScholarPubMed
Fincham, J. R. S. (1952). A comparative genetic study of the mating-type chromosomes of two species of Neurospora. J. Genet. 50, 221–229.CrossRefGoogle Scholar
Garnjobst, L. & Tatum, E. L. (1956). A temperature-independent riboflavin-requiring mutant of Neurospora crassa. Amer. J. Bot. 43, 149–157Google Scholar
Giles, N. H., De Serres, F. J. Jr. & Barbour, E. (1957). Studies with purple adenine mutants in Neurospora crassa. II. Tetrad analyses from a cross of an ad-3A mutant with an ad-3B mutant. Genetics, 42, 608–617.CrossRefGoogle ScholarPubMed
Gowans, C. S. (1960). Some genetic investigations on Chlamydomonas eugametos. Z. indukt. Abstamm.- u. Vererb Lehre, 91, 63–73.Google ScholarPubMed
Gross, S. R. & Fein, A. (1960). Linkage and function in Neurospora. Genetics, 45, 885–904.CrossRefGoogle ScholarPubMed
Hawthorne, D. C. & Mortimer, R. K. (1960). Chromosome mapping in Saccharomyces: Centromere-linked genes. Genetics, 45, 1085–1110.CrossRefGoogle ScholarPubMed
Heslot, H. (1958). Contribution à' l'étude cytogénétique et génétique des Sordariacées. Rev. Cytol. et. biol. végétales, 19, suppl. 2, 1–235.Google Scholar
Holliday, R. (1961). The genetics of Ustilago maydis. Genet. Res. 2, 204–230.CrossRefGoogle Scholar
Howe, H. B. Jr. (1956). Crossing over and nuclear passing in Neurospora crassa. Genetics, 41, 610–622.CrossRefGoogle ScholarPubMed
Käfer, E. (1958). An 8-chromosome map of Aspergillus nidulans. Advanc. Genet. 9, 105–145.CrossRefGoogle ScholarPubMed
Kitani, Y., Olive, L. S. & El-Ani, A. S. (1961). Transreplication and crossing over in Sordaria fimicola. Science, 134, 668–669.CrossRefGoogle ScholarPubMed
Kitani, Y., Olive, L. S. & El-Ani, A. S. (1962). Genetics of Sordaria fimicola. V. Aberrant segregation at the G locus. Amer. J. Bot. (in the press).Google Scholar
Knapp, E. (1960). Tetrad analyses in green plants. Canad. J. Genet. Cytol. 2, 89–95.CrossRefGoogle Scholar
Knapp, E. & Möller, E. (1955). Tetradenanalytische Auswertung eines 3-Punktversuchs bei Sphaerocarpus donnellii Aust. Z. indukt. Abstamm.- u. Vererb Lehre, 87, 298–310.Google Scholar
Leupold, U. (1958). Studies on recombination in Schizosaccharomyces pombe. Cold. Spr. Harb. Symp. quant. Biol. 23, 161–170.CrossRefGoogle ScholarPubMed
Leupold, U. & Hottinguer, H. (1954). Some data on segregation in Saccharomyces. Heredity, 8, 243–258.CrossRefGoogle Scholar
Levine, R. P. – Ebersold, W. T. (1958 a). The relation of calcium and magnesium to crossing over in Chlamydomonas reinhardi. Z. indukt. Abstamm.- u. Vererb Lehre, 89, 631–635.Google Scholar
Levine, R. P. & Ebersold, W. T. (1958 b). Gene recombination in Chlamydomonas reinhardi. Cold Spr. Harb. Symp. quant. Biol. 23, 101–109.CrossRefGoogle ScholarPubMed
Lewin, R. A. (1953). The genetics of Chlamydomonas moewusii. Gerloff. J.Genet. 51, 543–560.CrossRefGoogle Scholar
Lindegren, C. C. (1936). A six-point map of the sex-chromosome of Neurospora crassa. J. Genet. 32, 243–256.CrossRefGoogle Scholar
Lindegren, C. C. (1949). Chromosome maps of Saccharomyces. Proc. VIIIth Int. Cong. Genet., Hereditas Suppl. vol., 338–355.Google Scholar
Lindegren, C. C. & Lindegren, G. (1942). Locally specific patterns of chromatid and chromosome interference in Neurospora. Genetics, 27, 1–24.CrossRefGoogle ScholarPubMed
Lindegren, C. C. & Lindegren, G. (1951). Linkage relations in Saccharomyces of genes controlling the fermentation of carbohydrates and the synthesis of vitamins, amino acids and nucleic acid components. Indian Phytopath. 4, 11–20.Google Scholar
Lissouba, P. & Rizet, G. (1960). Sur l'existence d'une unité génétique polarisée ne subissant que des échanges non-réciproques. C. R. Acad. Sci., Paris, 250, 3408–3410.Google Scholar
Maling, B. D. (1959). The effect of environmental factors on crossing over in Neurospora crassa. Ph.D. Thesis, Stanford Univ. (Abstracted in Diss. Abstr. 20, 1135, (1959).)Google Scholar
Mitchell, M. B. (1956). A consideration of aberrant recombination in Neurospora. C. R. Lab. Carlsberg, ser. physiol. 26, 285–298.Google Scholar
Mitchell, M. B. (1960). Evidence of non-random distribution of ascus classes in fruiting bodies of Neurospora crassa. Genetics, 45, 1245–1251.CrossRefGoogle ScholarPubMed
Mitchell, M. B. & Mitchell, H. K. (1954). A partial map of linkage group D in Neurospora crassa. Proc. nat. Acad. Sci., Wash., 40, 436–440CrossRefGoogle Scholar
Monnot, F. (1953). Sur la localisation du gène S et sur quelques particularités du crossing-over chez Podospora anserina. C. R. Acad. Sci., Paris, 236, 2330–2332.Google Scholar
Murray, J. C. (1959). Studies of morphology, genetics, and culture of wild-type and morphological mutant strains of Neurospora crassa. Ph.D. Thesis, Cornell Univ. (Abstracted in Diss. Abstr. 20, 3480, (1960).)Google Scholar
Murray, N. E. (1960). The distribution of methionine loci in Neurospora crassa. Heredity, 15, 199–206.CrossRefGoogle Scholar
Papazian, H. P. (1951). The incompatibility factors and a related gene in Schizophyllum commune. Genetics, 36, 441–459.CrossRefGoogle Scholar
Papazian, H. P. (1952). The analysis of tetrad data. Genetics, 37, 175–188.CrossRefGoogle ScholarPubMed
Papazian, H. P. (1960). Cluster model of crossing over. Genetics, 45, 1169–1175.CrossRefGoogle ScholarPubMed
Perkins, D. D. (1962). Crossing over and interference in a multiply marked chromosome arm of Neurospora. (In preparation.)CrossRefGoogle Scholar
Pontecorvo, G. (1958). Trends in Genetic Analysis. New York: Columbia Univ. Press.Google Scholar
Pritchard, R. H. (1955). The linear arrangement of a series of alleles of Aspergillus nidulans. Heredity, 9, 343–371.CrossRefGoogle Scholar
Pritchard, R. H. (1960). Localized negative interference and its bearing on models of gene recombination. Genet. Res. 1, 1–24.CrossRefGoogle Scholar
Shult, E. & Lindegren, C. C. (1959). A survey of genetic methodology from Mendelism to tetrad analysis. Canad.J. Genet. Cytol. 1, 189–201.CrossRefGoogle Scholar
Stadler, D. R. (1956). A map of linkage group VI of Neurospora crassa. Genetics, 41, 528–543.CrossRefGoogle ScholarPubMed
Stadler, D. R. (1959 a). The relationship of gene conversion to crossing over in Neurospora. Proc. nat. Acad. Sci., Wash., 45, 1625–1629.CrossRefGoogle ScholarPubMed
Stadler, D. R. (1959 b). Gene conversion of cysteine mutants in Neurospora. Genetics, 44, 647–655.CrossRefGoogle ScholarPubMed
Strickland, W. N. (1958 a). Abnormal tetrads in Aspergillus nidulans. Proc. roy. Soc. B, 148, 533–542.Google ScholarPubMed
Strickland, W. N. (1958 b). An analysis of interference in Aspergillus nidulans. Proc. roy. Soc. B, 149, 82–101.Google ScholarPubMed
Stirickland, W. N. (1961). Tetrad analysis of short chromosome regions of Neurospora crassa. Genetics, 16, 1125–1141.CrossRefGoogle Scholar
Sturtevant, A. H. (1955). Evaluation of recombination theory. J. cell. comp. Physiol. 45 (Suppl. 2), 237–242.CrossRefGoogle ScholarPubMed
Weinstein, A. (1918). Coincidence of crossing over in Drosophila melanogaster (ampelophila). Genetics, 3, 135–172.CrossRefGoogle ScholarPubMed
Weinstein, A. (1957). Evidence that a short region cannot contain more than one exchange.Genetics, 42, 401–402.Google Scholar
Weinstein, A. (1958). The geometry and mechanics of crossing over. Cold Spr. Harb. Symp. quant. Biol. 23, 177–196.CrossRefGoogle ScholarPubMed
Wheeler, H. E. (1956). Linkage groups in Glomerella. Amer. J. Bot. 43, 1–6.CrossRefGoogle Scholar
Whitehouse, H. L. K. (1948). Genetics of ascomycetes. Ph.D.Thesis, Cambridge University. Quoted by Fincham (1952).Google Scholar
Whitehouse, H. L. K. (1956). The use of loosely-linked genes to estimate chromatid interference by tetrad analysis. C. R. Lab. Carlsberg, ser. Physiol. 26, 407–422.Google Scholar
Williams, E. B. & Shay, J. R. (1957). The relationship of genes for pathogenicity and certain other characters in Venturia inaequalis (Cke.) Wint. Genetics, 42, 704–711.CrossRefGoogle ScholarPubMed