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The fitness consequences of P element insertion in Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

Walter F. Eanes ,
Cedric Wesley ,
Jody Hey ,
David Houle  and
James W. Ajioka
Walter F. Eanes*
Affiliation:
Department of Ecology and Evolution, State University of New York, Stony Brook, New York 11794, U.S.A.
Cedric Wesley
Affiliation:
Department of Ecology and Evolution, State University of New York, Stony Brook, New York 11794, U.S.A.
Jody Hey
Affiliation:
Department of Ecology and Evolution, State University of New York, Stony Brook, New York 11794, U.S.A.
David Houle
Affiliation:
Department of Ecology and Evolution, State University of New York, Stony Brook, New York 11794, U.S.A.
James W. Ajioka
Affiliation:
Department of Ecology and Evolution, State University of New York, Stony Brook, New York 11794, U.S.A.
*
* Corresponding author.
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In this study we estimate the frequency at which P-element insertion events, as identified by in situ hybridization, generate lethal and mild viability mutations. The frequency of lethal mutations generated per insertion event was 0·004. Viability dropped an average of 1% per insertion event. Our results indicate that it is deletions and rearrangements resulting from the mobilization of P elements already in place and not the insertions per se that cause the drastic effects on viability and fitness observed in most studies of P–M dysgenesis-derived mutations. Elements of five other families (I, copia, 412, B104, and gypsy) were not mobilized in these crosses. Finally, we contrast the density of P elements on the X chromosome with the density on the four autosomal arms in a collection of thirty genomes from an African population. The relative number of P elements on the X chromosome is too high to be explained by either a hemizygous selection or a neutrality model. The possible reasons for the failure to detect selection are discussed.

Type
Research Article
Information
Genetics Research , Volume 52 , Issue 1 , August 1988 , pp. 17 - 26
Copyright
Copyright © Cambridge University Press 1988

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